Enzyme Kinetics Flashcards
1
Q
What do we need to know to understand how an enzyme works?
What are the 2 parameters used to describe this?
A
How the concentration of substrate - [S] affects the rate of the catalysed reaction - V.
“Vmax and Km”
2
Q
What is used to describe the relationship between Vmax and Km?
A
The Michaelis-Menten model
KM = k-1 + k2 / k1
3
Q
What does Vmax, KM
and what does KM =
A
Vmax is the maximum velocity of a reaction
KM is the concentration in moles of S which gives ½ Vmax
KM = [S] at 0.5 Vmax
