Enzyme kinetics

Question 1

Why are enzymes significant? (3 points)

Answer 1

As;

1. Enzymes are catalysts
2. Enzymes are specific
3. Enzymes control well defined chemical reactions

Question 2

To understand how an enzyme works what piece of information do we need to know?

Answer 2

We need to know how the concentration of substrate [S] affects the rate of the catalysed reaction (V).

Question 3

What two parameters are used to describe this relationship?

Answer 3

Vmax and KM

Question 4

What is the rate of the catalysed reaction (V) measured in?

Answer 4

Moles of substrate converted to product per second

Question 5

What is the concentration of substrate [S] measured in?

Answer 5

Moles

Question 6

What is the Michaelis-Menten model used to explain?

Answer 6

The Michaelis-Menten model is used to explain the relationship between Vmax and KM

Question 7

What do Enzyme reactions go like?

Answer 7

(enzyme)

substrate ——————> product

Question 8

What does k1 resemble?

Answer 8

k1 is the forward rate constant for enzyme association with the substrate.

Question 9

What does k-1 resemble?

Answer 9

k-1 is the backwards rate constant for enzyme dissociation from the substrate.

Question 10

What does k2 resemble?

Answer 10

k2 is the forward rate constant of enzyme conversion of substrate to product (P)

Question 11

What is the symbol for the Michaelis Constant?

Answer 11

KM

Question 12

What is the formula for KM?

Answer 12

k-1 + k2
KM = ————–
k1