Enzyme Kinetics Flashcards
1
Q
What is the equation for velocity (v)?
A
Vmax*(SUBSTRATE CONC / KM + SUBSTRATE CONC)
2
Q
What is Vmax?
A
Maximal velocity
3
Q
What is KM?
A
The substrate concentration that gives 1/2 the maximal velocity or Vmax/2.
4
Q
What are the equations for Vmax?
A
Vmax = 2KM
Vmax = kcat[Etot]
5
Q
What is [Etot]?
A
The enzyme concentration.
6
Q
What is kcat?
A
Essentially the rate at which the enzyme works (turnover number in a given time).
7
Q
What is KM?
A
Describes the enzyme’s substrate concentration dependence.
8
Q
On the graph, what does the Y intercept indicate?
A
The Y intercept = - 1/KM
9
Q
On the graph, what does the X intercept represent?
A
The X intercept = 1/Vmax
10
Q
After the addition of a competitive inhibitor, what happens to Vmax?
A
Vmax remains the same. KM changes.
