enzyme action Flashcards
during a chemical reaction
a new substance is formed as a substance changes from one form to the other
the substance of which the enzyme acts is known as
the substrate
enzymes
protein that has a particular shape to enable it to perform its speciifc function
enzymes are substrate specific meaning
a specific enzyme can only act on a specific substrate
active site
the part of the enzyme where substrate molecules bind and a chemical reaction takes place. The active site is made up of amino acid residues that establish temporary bonds with the substrate (binding site) as well as residues that catalyse that substrate’s reaction (catalytic site).
the substrate molecule fits into
the enzyme’s active site as a key fits into a lock to ensure the specificity of the enzyme then a temporary enzyme substrate complex is formed.
anabolic reaction in an active site
Anabolic reactions utilize energy to synthesize larger molecules from smaller units. Example- formation of glycogen from simple sugar molecules is an anabolic reaction.
temperature
enzymes are sensitive to temperature change. low temperatures make enzyme temporarily inactive .
the temperature at which enzymes work the best is known as their optimum temperature which is
35 to 40 degrees in humans (body temperature is 37)
at high temperatures
usually above 45 the shape of the enzyme begins to change and the substrate no longer fits into the active site. The enzyme can’t perform its function. the enzyme denatures
denaturation is an irreversible process
when the enzyme denatures it cannot return to its proper shape when the temperature is lowered again.When the enzyme becomes inactive as a result of low temperature it can become active again once the temperature rises.
optimum ph
each enzyme has a narrow ph range within which it can function. some enzymes qork optimally in acidic medium whereas others in a neutral or alkaline medium. enzymes denature at extreme ph levels
properties of enzymes
1) spherical proteins
2) sensitive to chnages in temperatures in high temperature the enzyme denatures whereas low tempratures to make temporarily inactive
3)substrate specific
$4)can be used over and over again
5) a small amount of enzyme can change a large amount of subtrate
enzymes in everyday life
biological washing powder such as bio tex/classic contain enzymes that break up/remove biological stains. these enzymes usually act on proteins and are known as protease stains caused by blood/sweats consist of difficult porteins to remove therefroe the enzyme in the washing powder break down the proteins down into smaller soluble molecules easily removed by cleaning agents
catalase
enzyme found in most animal and plant cells that accelerates the breakdown of hydrogen peroxides into water and oxygen. oxygen is released during this reaction as bubbles