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enzyme 4 Flashcards

1
Q

E.C. 3.1.1.3

A

LIPASE (LPS)

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2
Q

Responsible for triglyceride metabolism

A

LIPASE (LPS)

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2
Q

LIPASE (LPS) chemical name

A

Triacylglycerol Acylhydrolase

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2
Q

o Hydrolyzes glycerol esters of long chain fatty acids

A

LIPASE (LPS)

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2
Q

o most specific pancreatic marker

A

lipase

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2
Q

primary tissue in lipase

A

pancreas

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2
Q

lipase Expresses its full activity in the presence of

A

bile salts and colipase

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2
Q

substrate for cherry crandall

A

olive oil

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3
Q

substrate of vogel zieve

A

olive oil

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3
Q

reference method in titritmetric method

A

cherry crandall

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3
Q

titration is carried out by

A

potentiometry

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3
Q

 TISSUE SOURCES of lipase

A

Pancreas (1°source), GIT, leukocytes, adipose cells, colostrum

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3
Q

inhibitors of lipase

A

heavy metals, quinine & some esterase inhibitors

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3
Q

fatty acid is titrated w/ alkali solution

A

Titrimetric Methods

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3
Q

emulsion of fats produces milky appearance

A

Turbidimetric Methods

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3
Q

increases concentration of LPS

A

o Bacteria

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3
Q

lipase is not inhibited by

A

fluoride or arsenilate

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3
Q

based on the use of immunochromatography with monoclonal antibodies (meth of deter)

A

Urinary trypsinogen-2 test strip

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4
Q

substrate for modified cherry crandall

A

triolein

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4
Q

o incorporate sodium deoxycholate, CaCl2 , co-lipase

A

 Ziogenhean et. al. (Hoffman and Weiss)

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4
Q

 DuPont ACA Method substrate

A

triolein

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4
Q

 Fatty acid + chemical reagent

A

Fluorescein (4-methyl bellifuzone)

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4
Q

o Diffusion of enzyme into buffered agarose gel w/ deoxycholate & olive oil emulsion

A

 Rosital Diffusion Method

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4
Q

trypsin is Solely produced by

A

pancreatic acinar cells

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4
Q

Cleaves peptide bonds formed between the –COOH group of lysine or arginine with other amino acids

A

TRYPSIN

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4
Q

 Radio-immunoassay : makes use of

A

1251-labeled lipase

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4
Q

elevation in acute pancreatitis

A

 Perforated peptic ulcer & duodenal ulcer
 Intestinal obstruction
 Mesenteric Vascular obstruction

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4
Q

acute pancreatitis is not elevated in

A

mumps

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4
Q

E.C.3.4.21.4

A

trypsin

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5
Q

o Erlanson & Bergstrom substrate

A

Tributyrin

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5
Q

fatty acids are extracted using Petroleum ether

A

 Myrtle and Zell method

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5
Q

 Pancreas-specific serine protease

A

TRYPSIN

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5
Q

acute pancreatitis decreases in (henry)

A

8 to 14 days

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5
Q

acute pancreatitis decreases in (tietz)

A

7 to 14 days

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5
Q

currently being used for differentiating the cause of acute pancreatitis

A

 Trypsin assays

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5
Q

o more elevated in alcohol-associated pancreatitis

A

 Trypsinogen-2 & trypsin-2-AAT

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5
Q

more elevated in biliary pancreatitis

A

 Trypsinogen-1, AMS & LPS

5
Q

 E.C. 3.4.15.1

A

Angiotensin-Converting Enzyme (ACE)

5
Q

zymogen of trypsin

A

trypsinogen-1 & trypsinogen-2

5
Q

activator of trypsin

A

Enterokinase

5
Q

trypsin is Inactivated in plasma by

A

α-1-antitrypsin & α-2-macroglobulin

5
Q

Angiotensin-Converting Enzyme (ACE) converts

A

angiotensin I to angiotensin II

5
Q

o Diagnosis and monitoring sarcoidosis

A

Angiotensin-Converting Enzyme (ACE)

6
Q

 A.k.a peptidyl dipeptidase A / Kininase II

A

Angiotensin-Converting Enzyme (ACE)

6
Q

Angiotensin-Converting Enzyme (ACE) activity found in

A

lungs & endothelial cells

7
Q

 Inactivation of bradykinin, encephalin, tachykinin

A

Angiotensin-Converting Enzyme (ACE)

7
Q

Elevations are more likely in pulmonary involvement

A

Angiotensin-Converting Enzyme (ACE)

8
Q

E.C. 3.1.1.7

A

True Cholinesterase

9
Q

E.C. 3.1.1.8

A

Pseudocholinesterase

10
Q

Cleaves succinylcholine

A

Pseudocholinesterase or Acylcholine Acyl hydrolase

10
Q

muscle relaxant used during surgery

A

succinylcholine

11
Q

Can be found in Liver, myocardium & pancreas

A

Pseudocholinesterase or Acylcholine Acyl hydrolase

11
Q

Diagnosis of organophosphate insecticide poisoning

A

cholinesterase

11
Q

E.C. 3.4.11.1

A

Leucine Aminopeptidase (LAP)

12
Q

o True Cholinesterase uses

A

acetylcholine

12
Q

o Pseudocholinesterase uses

A

butyrylthiocholine

12
Q

 True Cholinesterase can be found in

A

o CNS, RBCs, Lung and Spleen

12
Q

o Released thiocholine reacts with

A

Ellman’s reagent

13
Q

True Cholinesterase (E.C. 3.1.1.7) & Pseudocholinesterase (E.C. 3.1.1.8) product is measured

A

photometrically

14
Q

major isoenzyme of LAP

15
Q

o Important in hydrolysis of Oxytocin & Angiotensin II

A

placental iso

15
Q

o Increased during 3rd trimester of pregnancy

A

placental iso

15
Q

LAP NV in males

A

19.2 – 48.0 IU/L

16
Q
  • Increased in obstructive liver diseases
16
Q

 Widely distributed; cell membrane-bound

A

5’-Nucleotidase

16
Q

 Increased 5’-NT (disease)

A

o Acute hepatitis
o Ovarian carcinoma
o Rheumatoid arthritis

16
Q

LAP NV in females

A

18.0 – 44.4 IU/L

16
Q

other name of 5’-Nucleotidase

A

 5’-ribonucleoside phosphohydrolase

17
Q

E.C.3.1.3.5

A

5’-Nucleotidase

17
Q

 Heme-containing protein that binds O2 with cardiac and skeletal muscle

17
Q

Myocardial infarction is diagnosed by the presence of 2 out of 3 features

A

a. Chest pain - prolonged
b. ECG changes
c. Cardiac enzymes

18
Q

 Levels are related to muscle mass and activity

18
Q

 Reasonable sensitivity but poor specificity for MI dx

19
Q

 Earliest marker in MI

19
Q

 Govern excitation-contraction coupling

19
Q

 Bind O2 within cardiac and skeletal muscle

20
Q

tropomyosin binding unit

A

o Troponin T (cTnT)

21
Q

calcium-binding subunit

A

o Troponin C (TnC)

22
Q

inhibitory subunit (binds to actin)

A

o Troponin I (cTnI)

23
Q

troponin sub units that are bound to muscle fibers

A

 cTnT and cTnI

24
Q

 cTnT and cTnI are bound to muscle fibers (weeks)

A

1–2 weeks after MI

24
Q

 “gold standard” for diagnosis of MI

25
Q

 False positive results in troponin

A

o Fibrin (incompletely clotted specimen)
o Rheumatoid Factor

25
Q

troponin specimen of choice

A

heparinized samples

26
Q

If both myoglobin and CA – III are increased it cause

A

skeletal muscle injury

26
Q

critical element in the dev’t of unstable angina & MI

A

 Thrombus formation

27
Q

 Protein marker found in skeletal muscle

A

CA – III (CA Isoenzyme III)

28
Q

CA – III (CA Isoenzyme III) is not found in

A

cardiac muscle

29
Q

diagnostic test of choice for recognition of cardiac injury

30
Q

Markers for Cardiac Muscle injury existing with Skeletal Muscle injury

A

 Total CK, AST, Myoglobin

CC2 (5 decks)

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