DRUG OF ABUSE Flashcards
1
Q
TLC
(Thin Layer Chromatography)
sensitivity
A
0.2-5
2
Q
- Uses enzyme-labeled drugs that compete with the drug in the sample
A
EMIT
2
Q
EIA sensitivity
A
0.025-5
2
Q
- Separates bound drugs from unbound drugs
A
ELISA
2
Q
GC sensitivity
A
0.01-10
2
Q
HPLC sensitivity
A
0.02-10
2
Q
GCMS sensitivity
A
0.001-5
3
Q
- More rapid than RIA
A
EMIT
3
Q
law for drig testing
A
- Comprehensive Dangerous Drugs Act of 2002 (R.A. 9165)
3
Q
- Fluorescent product
A
Umbelliferone
3
Q
- Drug binds to a fluorogenic substrate
A
Fluorescence Immunoassay
3
Q
- Serum is incubated with an antibody and a radiolabeled drug, competing for antibody binding sites.
A
RIA
3
Q
- Uses specific antibodies bound to a solid-state carrier
A
ELISA
3
Q
two fractions of RIA
A
1) Free fraction (unbound drug)
2) Antibody-bound drug
3
Q
- Adsorption of drug to a solid support and elution by means of a mobile and liquid phase
A
chromatography
3
Q
screening for drug identification
A
TLC
4
Q
for confirmatory drug ID
A
GCMS
4
Q
- Urine sample adjusted to ph 8.5
A
TLC
