Enumeration of Bacteria/Bacteriophage Titer

Question 1

analyzing population growth

Answer 1

turbidimetry; cloudy = bacterial growth
spectrophotometry; can read absorbance of light through bacteria culture (indirect measurement technique so cannot determine cells alive vs dead)

Question 2

IMPORTANT 2 Qson calculating serial dilutions

Answer 2

colonies on plate x dilution factor = CFU per mL of og sample

Question 3

bacteriophage dilution series

Answer 3

soft agar + 3 drops of e. coli
1 mL into 9 mL to make 10 mL full dilution
continuously transfer 1mL to next tube
(can determine alive cell count)

Question 4

each colony

Answer 4

originates from a different cell; each colony represents one bacterial cell present in diluted sample