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GRAM NEGATIVE COCCI AND GRAM NEGATIVE BACILLI > ENTEROBACTERIACEAE > Flashcards

ENTEROBACTERIACEAE Flashcards

1
Q

Enterobacterias are commensal floras of the…

A

Intestinal tract

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2
Q

Enterobacterias that are not part of a normal flora

A

Salmonella
Shigella
Yersinia

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3
Q

Microscopic appearance of Enterobacterias

A

Gram-negative straight rods or coccobacilli with rounded ends

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4
Q

Culture morphology of Enterobacterias

A

Large, smooth, gray colonies (except Klebsiella and Enterobacter)
Non hemolytic (except E. Coli - beta hemolytic)

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5
Q

Characteristics of Enterobacteriaceae

A

Gram negative straight rods or coccobacilli
Non hemolytic (except E. Coli - beta hemolytic)
Motile (except Klebsiella, Shigella, and Yersinia)
Non encapsulated (except Klebsiella, and Enterobacter)
Glucose fermenter
Reduce nitrate to nitrite
Some may grow at low temperature: 1-5ºC (Serratia and Yersinia)

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6
Q

Non motile Enterobacteriaceae

A

“SKY”

Shigella
Klebsiella
Yersinia

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7
Q

Motility organelle used by Enterobacterias

A

Peritrichous flagella

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8
Q

Encapsulated Enterobacterias

A

Klebsiella
Enterobacter

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9
Q

Enterobacterias that grow at low temperatures (1-4ºC)

A

Yersinia
Serratia

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10
Q

Gram negative cocci
Catalase (+)
Cytochrome oxidase (+) (except Plesiomonas shigelloides)
Alginate liquefaction (+)

A

Enterobacteriaceae

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11
Q

Opportunistic Enterobacteriaceae pathogen

A

“PECKS”

Proteus
Enterobacter
Citrobacter
Klebsiella
Serratia

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12
Q

Overt/true Enterobacteriaceae pathogen

A

Salmonella typhi
Shigella
Yersinia pestis

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13
Q

They are part of the intestinal microbiota of both humans and animals but may produce serious extraintestinal diseases when outside their normal body sites

A

Opportunistic pathogens

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14
Q

They are not present as commensal flora of the GIT of humans and only inhabit the bowel at the time of infection. They are generally acquired through ingestion of contaminated food or water.

A

Overt/True pathogens

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15
Q

Extended-spectrum-B-lactamase-producing Enterobacterias

A

“EKKstended-…”

Escherechia coli
Klebsiella pneumonia
Klebsiella oxytoca

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16
Q

Isolated from urinary tract and cause bacteremia

A

“PEK”

Proteus mirabilis
Escherichia coli
Klebsiella pneumoniae

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17
Q

Antibiotic-resistant genera of Enterobacteriaceae

A

“SEC”

Serratia
Enterobacter
Citrobacter

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18
Q

Enterobacteria associated with diarrhea

A

“YESS”

Yersinia
Escherichia coli
Salmonella
Shigella

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19
Q

Antigen determinants used for serological identification of Enterobacterias

A

“O” antigen
“H” antigen
“K” antigen

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20
Q

“O” antigen or…

A

Somatic antigen

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21
Q

“H” antigen or…

A

Flagellar antigen

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22
Q

“K” antigen or…

A

Capsular antigen

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23
Q

Heat stable antigen; located in the cell wall

A

“O” antigen

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24
Q

Heat labile antigen; found in the flagellum

A

“H” antigen

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25
Q

Heat labile polysaccharide; covers the O antigen

A

“K” antigen

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26
Q

Which bacterias are serotyped with “O” antigens

A

Escherichia coli
Shigella

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27
Q

Which bacterias are serotyped with “H” antigen

A

Salmonella

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28
Q

Which bacterias are serotyped with “K” antigen

A

Escherichia coli
S. enterica subsp. enterica serotype Typhi

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29
Q

Possesses “K1” capsular antigen

A

E. coli

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30
Q

Possesses “Vi” capsular antigen

A

S. enterica subsp. enterica serotype Typhi

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31
Q

Aka colon bacillus

A

Escherichia coli

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32
Q

Where is Escherichia coli found in humans?

A

Normal bowel flora
Female genital tract

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33
Q

It invades enterocytes lining the large intestine

A

Escherichia coli

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34
Q

It is a primary marker of fecal contamination in water purification

A

Escherichia coli

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35
Q

It is the leading cause of nosocomial infection — UTI

A

Escherichia coli

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36
Q

Flat, dry, with pink colonies (MAC)
Beta hemolytic (BAP)
Some strains may be NLF or LF
Greenish metallic sheen (EMB)

A

Escherichia coli

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37
Q

E. coli “O” groups have shown cross reactivity with ___________

A

“O” antigens of Shigella

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38
Q

Antigenic determinants on Escherichia coli

A

O, H, K antigens

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39
Q

Escherichia coli virulence factors

A

“ECoKI” ~sounds like E. coli~

Endotoxin
Common pili
K1 antigen
Intimin

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40
Q

Escherichia coli virulence factor responsible for neonatal meningitis

A

K1 antigen

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41
Q

Identical to the capsular antigen on Neisseria meningitidis group B

A

K1 antigen

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42
Q

Escherichia coli IMVIC and TSI reaction

A

IMVIC: + + - -
TSI: A/A, (+) gas, (-) H2S

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43
Q

Formerly called E. coli atypical or enteric group II

A

Escherichia hermanii

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44
Q

Escherchia with yellow pigmented colonies

A

Escherichia hermanii

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45
Q

E. coli strain that does not produce toxin and only attaches to the brush border of the intestinal epithelial cells causing cell damage

A

Enteropathogenic E. coli

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46
Q

E coli strain causing infantile diarrhea (stool without blood)

A

Enteropathogenic E. coli

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47
Q

E coli strain with H antigen and intimin

A

Enteropathogenic E. coli

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48
Q

Colonizes the proximal small intestine, causing Traveler’s diarrhea or montezuma’s revenge

A

Enterotoxicogenic E coli

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49
Q

Persons with achlorhydia are at risk of getting this E coli strain

A

Enterotoxicogenic E coli

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50
Q

Enterotoxicogenic E coli virulence factor

A

Heat stable (ST) and heat-labile (LT) enterotoxin

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51
Q

Infective dose of Enterotoxicogenic E coli

A

10^6 to 10^10

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52
Q

E coli strain that penetrates and multiplies within the intestinal epithelial cells, causing dysentery-like or shigella-like infections

A

Enteroinvasive E coli

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53
Q

Infection with this E coli strain produces watery diarrhea with WBC

A

Enteroinvasive E coli — dysentery-like or shigella-like infection

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54
Q

Enteroinvasive E coli virulence factor

A

Invasin

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55
Q

E. coli strain positive for Sereny test

A

Enteroinvasive E coli

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56
Q

Infective dose of Enteroinvasive E coli

A

10^6

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57
Q

Associated with hemolytic uremic syndrome an thrombotic thrombocytopenia purpura

A

Enterohemorrhagic E coli serotype 0157:H7

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58
Q

What strain and its toxin is responsible for the destruction of vascular endothelial cells which cause hemorrhagic colitis, and hemolytic uremic syndrome (HUS)?

A

Verotoxin present in Enterohemorrhagic E coli

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59
Q

Shiga-like toxin

A

Verotoxin

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60
Q

Causes infection with bloody diarrhea (without WBC)

A

Enterohemorrhagic E coli

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61
Q

Shigella-like toxin E coli

A

Enteroinvasive E coli
Enterohemorrhagic E coli

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62
Q

E coli strain with (+) intimin; (-) MUG

A

Enterohemorrhagic E coli

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63
Q

2 types of Enteroadherent E coli

A

Enteroadherent E coli (EAEC)
Diffusely adherent E coli (DAEC)

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64
Q

E coli strain that adheres to the surface of the intestinal mucosa and causes watery diarrhea, or UTI

A

Enteroadherent E coli

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65
Q

E coli strain that adheres to Hep2 cells forming clumps of bacteria and causes UTIs

A

Diffusely adherent E coli

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66
Q

Enteroadherent E coli virulence factor

A

Fimbriae

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67
Q

Causes “stacked-brick” appearance of clumped bacterias

A

Diffusely adherent E coli

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68
Q

Most common cause of UTIs in humans

A

Uropathogenic E coli

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69
Q

Uropathogenic E coli virulence factor

A

“PAC”

Pili
Aerobactin
Cytolysin

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70
Q

A test used to test the invasiveness of enteroinvasive Escherichia coli, Shigella species, and Listeria monocytogenes. It is done by inoculating suspension of bacteria into guinea pig’s eye. Severe mucopurulent conjunctivitis and severe keratitis indicates a positive test.

A

Sereny test

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71
Q

Bacterial adhesion molecule involved in attachment of enteropathogenic and enterohaemorrhagic Escherichia coli to mammalian host cells

A

Intimin

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72
Q

Vibrio-like E coli

A

Enterotoxigenic E coli

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73
Q

___________ activates guanylate cyclase which stimulates the production of __________

A

Heat-stable enterotoxin (ST)
Cyclic guanosine monophosphate (cGMP)

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74
Q

Induces the secretion of electrolytes and water into the lumen of the intestine resulting to watery diarrhea

A

Cyclic guanosine monophosphate (cGMP)
Cyclic adenosine monophophate (cAMP)

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75
Q

_________ binds to specific gangliosides on the epithelial cells and activates membrane bound adenylate cyclase, which leads to increased production of ________

A

Heat-labile enterotoxin (LT)
Cyclic adenosine monophosphate (cAMP)

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76
Q

AKA Shiga toxicogenic E. coli

A

Enterohemorrhagic E. coli

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77
Q

Biochemical characteristics of Klebsiella

A

KCN (+)
H2S (-)
MR (-)
VP (+)

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78
Q

Friedlander’s bacillus

A

Klebsiella pneumoniae subsp. pneumoniae

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79
Q

Formerly known as the bacillus capsulatus

A

Klebsiella pneumoniae subsp. pneumoniae

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80
Q

The most common isolated spp of Klebsiella

A

Klebsiella pneumoniae subsp. pneumoniae

81
Q

Causative agent of community acquired pneumonia

A

Klebsiella pneumoniae subsp. pneumoniae

82
Q

Causes currant jelly-like sputum

A

Klebsiella pneumoniae subsp. pneumoniae

83
Q

It is a frequent cause of lower respiratory tract infections among hospitalized patients and in immunocompromised hosts

A

Klebsiella pneumoniae subsp. pneumoniae

84
Q

Pink, mucoid colonies (MAC)

Neufeld Quellung test (+)
TSI: A/A, (+) gas, (-) H2S
IMVIC (- - + +)
KCN (+)
Urease (+)
Lysine decarboxylase (+)
Gelatin @ 22ºC (-)
Na alginate utilization (+)

A

Klebsiella pneumoniae subsp. pneumoniae

85
Q

Virulence factor of Klebsiella pneumoniae subsp. pneumoniae

A

Polysaccharide capsule

86
Q

Differential test for Klebsiella pneumoniae subsp. pneumoniae

A

String test (+)

87
Q

Causes chronic atrophic rhinitis (“foul smelling” atrophic rhinitis)

A

Klebsiella pneumoniae subsp. ozanae

88
Q

Causes a granuloma of the nose and oropharynx

A

Klebsiella pneumoniae subsp. rhinoscleromatis

89
Q

Klebsiella species that are indole positive

A

K. oxytoca
K. ornithinolytica

90
Q

Biochemical test:

IMVIC (+ + + +)
Urease (+)
Lysine decarboxylase (+)
Gelatin @ 22ºC (-)

A

Klebsiella oxytoca

91
Q

Resembles Klebsiella on MAC: pink colonies and maybe mucoid colonies

A

Enterobacter

92
Q

Has been isolated with osteomyelitis following traumatic wounds

A

Enterobacter cancerogenous

93
Q

A pathogen in neonates causing meningitis and bacteremia, often coming from powdered infant formula

Culture: yellow-pigmented colonies

A

Cronobacter sakazaki

94
Q

Causes nosocomial outbreak of septicemia due to contaminated IV fluids. It shows “triple decarboxylase negative” reaction

A

Pantoea agglomerans

95
Q

Musty-pungent odor or “potato-like” odor

A

Serratia odoriferae

96
Q

Most clinically significant Serratia spp.

A

Serratia marcescens

97
Q

Causes bacteremic outbreaks in nurseries and cardiac surgery and burn units. It is a contaminant in antiseptic solutions used for joint injections causing an epidemic of septic arthritis

A

Serratia marcescens

98
Q

Causes osteomyelitis following a motorcycle accident

A

Serratia plymuthica

99
Q

It is not known to cause gastroenteritis but is occasionally isolated from stool cultures. It has a distinctive feature: delayed citrate reaction.

A

Hafnia alvei

100
Q

It can infect the proximal kidney tubules and can cause AGN particularly in patients with urinary tract defects or catheterization.

A

Proteus

101
Q

Culture:

“swarming phenomenon” and “burn chocolate” or “burnt-gun powder” odor

A

Proteus

102
Q

Common isolates of Proteus

A

Proteus mirabilis

103
Q

Proteus that is a human pathogen

A

Proteus mirabilis
Proteus vulgaris

104
Q

TRUE OR FALSE

The swarming phenomenon is a result of a tightly regulated cycle of differentiation from standard vegetative cells (swimmers) to hyperflagellated, elongated, polyploidy cells (swarmers) capable of coordinated surface movement

A

TRUE :’)

105
Q

Is a rapid urease producer which encourages renal stone formation (urea splits in urine = increased urine pH). It is isolated from urine, wound, and ear infections.

A

Proteus

106
Q

Biochemical characteristics of Proteus

A

PAD test (+)
LIA (R/A)
IMVIC: (- + v v) P mirabilis; (+ + - v) P vulgaris
TSI: K/A, (+) gas, (+) H2S - P mirabilis
K/A, (+/-) gas, (+) H2S - P vulgaris

107
Q

One of the causes of nosocomial outbreaks involving burn units.
PAD test (+)
IMVIC (+ + - +)
TSI: K/A, (-) gas, (-) H2S

A

Providencia

108
Q

A pathogen of the urinary tracts that also causes diarrheal disease among travelers

A

Providencia rettgeri

109
Q

It has been isolated from noscomial outbreaks in burn units and in urine cultures. It is mostly resistant to antimicrobial agents (together with P rettgeri)

A

Providencia stuartii

110
Q

Providencia spp most commonly found in the feces of children with diarrhea

A

Providencia alcalifaciens

111
Q

Differentiates P rettgeri from P stuartii

A

Christensen’s urea: (+) P rettgeri ; (-) P stuartii

112
Q

Biochemical characteristics of Morganella

A

Same biochemical reaction with P vulgaris except citrate (-)
PAD test (+)
LIA (R/A)
IMVIC (+ + - -)
TSI: K/A, (+/-) gas, (+) H2S

113
Q

Edwardsiella spp pathogenic to humans

A

Edwardsiella tarda

114
Q

It has been isolated from cold-blooded and warm blooded animals
Biochemical test:
Urease (-)
LDC (+)
IMVIC ++—
TSI K/A, (+) gas, (+) H2S

A

Edwardsiella

115
Q

A plant pathogen enterobacteriaceae not significant in human infections

A

Erwinia

116
Q

Colonies on MAC resemble E coli and biochemically resembling Salmonella. It can cause false (+) agglutination test with salmonella

A

Citrobacter

117
Q

Has been associated with endocarditis in IV drug users. The colony morphology resembles Salmonella when isolated from stool cultures.

A

Citrobacter freundii

118
Q

It produces type 1 cephalosporinase

A

Citrobacter freundii

119
Q

It causes nursery outbreaks of neonatal meningitis and brain abscesses

A

Citrobacter koseri

120
Q

The most serous pathogenic Enterobacteria for humans

A

Salmonella

121
Q

An inhabitant of the GI tract of animals. Humans acquire this organism by ingestion of contaminated animal products or improperly cooked poultry, milk, eggs, and dairy products. It may also be transmitted by “human carriers”

A

Salmonella

122
Q

Salmonella biochemical characteristics

A

Motile (except S pullorum and S gallinarum)
Gas (+) (except S gallinarum and S typhi)
H2S (+) (except S paratyphi A)
LDC (+)
KCN (-)
IMVIC (-+-+ / -+—) S typhi
TSI: K/A, (+/-) gas, (+) H2S

123
Q

Salmonella virulence factor

A

Fimbriae
Enterotoxin

124
Q

Main etiologic agent of enteric fever

A

Salmonella typhi

125
Q

Etiologic agents of paratyphoid fever

A

Salmonella paratyphi A, B, C
Salmonella choleraesuis

126
Q

Salmonella isolated from lizards and other cold-blooded animals

A

Salmonella bongori

127
Q

Most common Salmonella strain associated with gastroenteritis delete

A

Salmonella enterica subsp. enterica

128
Q

Causes peanut-butter outbreak

A

Salmonella typhimurium

129
Q

Diseases caused by Salmonella

A

Gastroenteritis
Typhoidfecer
Bacteremia

130
Q

Gastroenteritis is one of the most common forms of “food poisoning.” This is spread by using contaminated cooking utensils. Inadequate refrigeration also allows the growth and multiplication of the organism. Poultry, eggs, milk, and handling of pets are the main source of infection. Symptoms include nausea, vomiting, fever and chills, watery diarrhea and abdominal pain.

What is the causative agent?

A

Salmonella enterica subsp enterica

131
Q

Infective dose of Salmonella enterica

A

10^6

132
Q

A febrile disease that results from the ingestion of contaminated food originating from infected individual or carriers, contaminated food and water, and fomites. Outbreaks can also be caused by improper disposal of sewage, poor sanitation and lack of modern water system. Symptoms include malaise, anorexia, lethargy, myalgia, and continuous frontal dull headache.

A

Enteric fever/typhoid fever

133
Q

Causative agent of typhoid fever

A

Salmonella typhi

134
Q

Characteristic papules that appear during the second week of typhoid fever

A

“Rose spot” blanching rose-colored papules that appear around the periumbilical region

135
Q

The site of long-term carriage of Salmonella typhi for individuals who have recovered from the infection

A

Gall bladder

136
Q

Causes necrotizing cholecystitis and Peyer’s patches

A

Salmonella typhi

These are the complications of typhoid fever

137
Q

Salmonella resistant to gastric acids and, on reaching the proximal end of the small intestine, invades and penetrates the intestinal mucosa

A

Salmonella typhi

138
Q

Characterized by prolonged fever and intermittent bacteremia. This occurs with and without extraintestinal foci of infection caused by nontyphoidal Salmonella.

A

Bacteremia

139
Q

Salmonella responsible for bacteremia

A

Non typhoidal salmonella: S typhimurium, S paratyphi, and S cholerasuis

140
Q

Treatment for chronic state of enteric carriers

A

Cholecystectomy is the only remedy

141
Q

What specimen for salmonella id is collected for the 1st week of infection?

A

Blood

142
Q

What specimen for salmonella id is collected for the 2nd week of infection?

A

Stool

143
Q

What specimen for salmonella id is collected for the 3rd week of infection?

A

Urine

144
Q

Closely related to genus Escherichia. It is an intracellular organism that multiplies in the cells of the colon epithelium.

A

Shigella

145
Q

Mode of acquisition of Shigella

A

Flies, fingers, food and feces (the four F’s) and water by infected persons (fecal-oral route)

146
Q

Culture:

Clear, fragile, NLF colonies
colorless colonies without black centers (SSA)

A

Shigella

147
Q

Shigella reservoirs

A

Humans only

148
Q

Most virulent Shigella species

A

Shigella dysenteriae

149
Q

Gay bowel syndrome

A

Shigella flexneri

150
Q

Shigella virulence factor

A

Shiga toxin

151
Q

Shigella antigenic structures

A

Somatic O

152
Q

Shigella specimen

A

Rectal swab

153
Q

Shigella biochemical characteristics

A

Non motile
Mannitol fermenter (except Shigella dysenteriae)
Does not utilize acetate or mucate as source of carbon
(-) gas from glucose (except Shigella flexneri)
Urease (-)
LDC (-)
IMVIC (v+—)
TSI: K/A, (-) gas, (-) H2S

154
Q

Susceptible to disinfectants and high concentrations of acids and bile

A

Shigella

155
Q

Why are Shigella specimens plated immediately after collection

A

Shigella is sensitive to pH changes (susceptible to acidic stool)

156
Q

Is unique in its ability to decarboxylate ornithine; late lactose fermenter; ONPG (+)
Infection from this organism is self-limiting and usually characterized by fever and watery diarrhea

A

Shigella sonnei

157
Q

Most common causative agent of bacillary dysentery

A

Shigella dysenteriae type 1

158
Q

Characterized by acute inflammatory colitis and bloody diarrhea (blood mucus, and WBCs) caused by the penetration of the intestinal epithelial cells by the organism, following attachment of the organisms to mucosal cell. Symptoms include fever, chills, abdominal cramps, painful bowel movement, and tenesmus.

A

Bacillary dysentery

159
Q

Its presence usually indicates improper sanitary conditions and poor personal hygiene

A

Shigella dysenteriae type 1

160
Q

Infective dose of Shigella dysenteriae

A

<200 bacilli

It is highly communicable because of its low infective dose required to produce the disease

161
Q

AKA plague bacillus

A

Yersinia pestis

162
Q

Yersinia pestis is considered as a ________ bioterrorism agent

A

Class A bioterrorism agent

163
Q

The only Enterobacteriaceae transmitted to humans by the bite of an infected flea

A

Yersinia pestis

164
Q

Vector of transmission for Yersinia pestis

A

Xenopsylla cheopis

165
Q

Where do Yersinia pestis multiply?

A

Inside phagocytes

166
Q

Protein responsible for the down-regulation of anti-bacterial responses of the host which allows Yersinia pestis to survive and multiply inside phagocytes

A

Yersinal plasmid-encoded outer membrane proteins (YOPS)

167
Q

Microscopic appearance of Yersinia pestis

A

Short, plump rod with “bipolar staining or closed safety pin appearance”

168
Q

Stain used for Yersinia pestis

A

Wayson or methylene blue stain

169
Q

Culture morphology of Yersinia pestis

A

Pinpoint colonies; grows best at 25º-30º C
Stalactite pattern (broth culture)

170
Q

Yersinia pestis virulence factor

A

Endotoxin
Coagulase
Fibrinolysin

171
Q

Mode of transmission of plague

A

Is a disease of the rodents transmitted to humans by fleas
Ingestion of contaminated animal tissues
Inhalation of contaminated airborne droplets

Once inside the human body, the bacteria multiplies in the blood and lymph

172
Q

2 forms of plague

A

Bubonic plague
Pulmonary plague

173
Q

Associated with high fever and painful inflammatory swelling of axilla and groin. It results from the bite of an infected flea

A

Bubonic plague

174
Q

Occurs secondary to the bubonic plague. It is acquired by close contact with other victims.

A

Pulmonary plague

175
Q

The most commonly isolated Yersinia

A

Yersinia enterocolitica

176
Q

Causative agent of waterborne gastroenteritis

A

Yersinia enterocolitica

177
Q

How is Yersinia enterocolitica isolated from a stool specimen?

A

Requires cold enrichment technique (4ºC) using phosphate buffered saline for several weeks

178
Q

Biochemical characteristics of Yersinia enterocolitica

A

Motile at 22ºC but not at 35ºC
Ferments sucrose and mannitol
IMVIC (v+—)
TSI: K/A, (-) gas, (-) H2S

179
Q

Microscopic appearance of Yersinia enterocolitica

A

Coccobacilli with bipolar staining

180
Q

Culture morphology of Yersinia enterocolitica

A

“Bull’s eye colonies” (dark red or burgundy centers with transparentborders) in CIN medium

181
Q

Optimum temperature for Yersinia enterocolitica

A

25º-30ºC

182
Q

Selective medium for Yersinia enterocolitica

A

Cefsulodi-irgasan-novobiocin (CIN) agar

183
Q

Yersinia enterocolitica reservoir

A

Swine
Dogs
Cats
Rabbits
Cattle

184
Q

Infections caused by Yersinia enterocolitica

A

Enterocolitis — waterborne gastroenteritis
Appendicitis-like syndrome
Arthritis
Erythema nodosum

185
Q

Yersinia enterocolitica mode of acquisition

A

Consumption of incompletely cooked food and dairy products, and handling pets

186
Q

A pathogen of rodents, particularly guinea pigs

A

Yersinia pseudotuberculosis

187
Q

Yersinia pseudotuberculosis reservoir

A

Farm and domestic animals (birds)

188
Q

Yersinia pseudotuberculosis mode of acquisition

A

Close contact with infected animals on their fecal material and ingestion of contaminated drink and food

189
Q

Biochemical characteristic of Yersinia pseudotuberculosis

A

Motile at 18º-25ºC
Urease (+)
Ferments rhamnose

190
Q

At what temperature is Yersinia pseudotuberculosis motile?

A

18º-25ºC

191
Q

At what temperature is Yersinia enterocolitica motile?

A

22ºC but not at 35ºC

192
Q

Antigens present in Yersinia enterocolitica

A

V and W antigens

193
Q

At what temperature is Yersinia pestis motile?

A

They are nonmotile

194
Q

The only oxidase (+) member of Enterobacteriaceae

A

Plesiomonas shigelloides

195
Q

Microscopic appearance of Plesiomonas shigelloides

A

Gram negative straight bacilli; occurs singly, in pairs, short chains or filamentous forms

196
Q

Culture morphology of Plesiomonas shigelloides

A

Shiny, opaque, smooth, and nonhemolytic (BAP)
white to pink colonies (Inositol brilliant green bile salt agar)
Opaque “apron-like colonies” (CIN)
(-) growth on TCBS
(+) growth on HEA

197
Q

Plesiomonas shigelloides morphology on CIN

A

Opaque “apron-like” colonies

198
Q

Often cross-agglutinates with Shigella

A

Plesiomonas shigelloides

199
Q

Plesiomonas shigelloides antigenic structures

A

Somatic O
Flagellar H (polar flagella)

GRAM NEGATIVE COCCI AND GRAM NEGATIVE BACILLI (5 decks)

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