Embryology of the Lungs Flashcards

1
Q

During what week does the respiratory diverticulum appear?

A
  • the respiratory diverticulum appears as an outpouching from the ventral wall of the foregut during week 4
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2
Q

What substance and transcription factor are important in determining the location and appearance of the lung bud (respiratory diverticulum)?

A
  • appearance and location of the lung bud are dependent on an increase in retinoic acid produced by adjacent mesoderm
  • an increase in RA upregulates TBX4 expressed in the endoderm of the gut tube at the site of the lung bud
  • TBX4 induces formation of the lung bud and continued growth and differentiation of the lungs
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3
Q

From which germ cell layer are the lungs developed from?

A
  • the epithelial lining of the larynx, trachea, bronchi and lungs is of endodermal origin
  • the cartilagenous, muscular and connective tissue components of the trachea and lungs are derived from splanchnic mesoderm surrounding the foregut
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4
Q

On what day does the lung bud appear?

How does it grow and what separates it from the foregut?

A
  • it appears on day 22 and is initially in open communication with the foregut
  • as it grows ventrocaudally, 2 longitudinal tracheo-oesophageal ridges form to separate the respiratory diverticulum from the foregut
  • the lung bud remains in communication with the laryngeal inlet
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5
Q

What happens to the tracheosophageal ridges after they have formed?

A
  • the tracheoesophageal ridges fuse to form the tracheoesophageal septum
  • this divides the foregut into a dorsal portion (oesophagus) and ventral portion (trachea and lung buds)
  • the respiratory diverticulum then divides to form 2 lung buds
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6
Q

What is a tracheosophageal fistula and why does it occur?

A
  • TOFs result from incomplete division of the foregut into oesophageal and respiratory portions
  • abnormalities in partitioning of the oesophagus and trachea by the tracheoesophageal septum results in oesophageal atresia +/- TOFs
  • a fistula describes an abnormal connection
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7
Q

What are TOFs associated with in 90% of cases?

A

closed or absent oesophageal atresia

  • the upper portion of the oesophagus ends in a blind pouch
  • the lower segment of the oesophagus forms a fistula with the trachea
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8
Q

What 2 variations of TOFs each account for 4% of cases?

A
  • isolated oesophageal atresia where there is no formation of a fistula
  • H-type TOF without oesophageal atresia
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9
Q

What is the most common complication of TOF with oesophageal atresia?

A
  • the upper oesophagus ends rapidly and lower oesophagus forms a fistula with the trachea
  • the abdomen rapidly distends as the stomach fills with air
  • it can also cause aspiration pneumonia as milk can enter the trachea from the blind-ended sac
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10
Q

What is the clinical consequence of a H-type TOF?

A
  • this can lead to milk being “driven” into the respiratory system
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11
Q

What other congenital abnormalities are TOFs usually associated with?

A
  • they are most commonly associated with cardiac defects (1/3 of cases)
  • they are part of the VACTERL association - a collection of defects with unknown causation but occur more frequently than predicted by chance alone
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12
Q

What is the first stage in formation of the lungs?

What happens during week 5 of development?

A
  • during partitioning of the oesophagus and the lung bud, right and left bronchial buds form
  • further growth and differentiation at the start of week 5 leads to enlargement of the bronchial buds to form right and left main bronchi
  • the right main bronchus forms 3 secondary bronchi
  • the left main bronchus forms 2 secondary bronchi
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13
Q

How do the secondary bronchi further divide during week 6 of development?

A
  • further branching results in the formation of tertiary bronchi, which will each supply a bronchopulmonary segment
  • there are 10 tertiary bronchi on the right and 8 on the left
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14
Q

Following the formation of tertiary bronchi, what further divisions occur?

How is this branching regulated?

A
  • branching continues to form terminal bronchioles by week 16 and respiratory bronchioles by week 26
  • the first alveoli develop in week 36
  • branching is regulated in part by the interaction of the epithelium (derived from the foregut) with the overlying visceral mesoderm
  • the visceral mesoderm forms the cartilage, smooth muscle, connective tissue and capillaries
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15
Q

What forms the 2 different types of pleura?

A
  • visceral mesoderm forms the visceral pleura
  • parietal mesoderm forms the parietal pleura
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16
Q

What is pulmonary agenesis and why does it occur?

A
  • it occurs when the lung bud fails to split, leading to complete absence of bronchi and vasculature
  • it can be unilateral or bilateral
  • bilateral pulmonary agenesis is incompatible with life
17
Q

What is the typical clinical presentation of unilateral pulmonary agenesis?

A
  • child usually develops respiratory distress if the remaining lung is compromised (usually by LRTI)
  • 60% have other congenital abnormalities including:
    • diaphragmatic hernias
    • cardiac lesions
    • skeletal abnormalities
  • agenesis of the right lung is associated with a higher frequency of anomalies
18
Q

Why can the presentation of pulmonary agenesis be variable?

A
  • the severity depends on the area of tissue affected - a single lobe or a whole lung?
  • clinical features vary from asymptomatic to respiratory complaints such as:
    • dyspnoea
    • recurrent pulmonary infections
    • respiratory distress
    • limited exercise tolerance
19
Q

How would an X-ray and endoscopic view confirm pulmonary agenesis?

A
  • there would be enlargement of the lung that is present and deviation of the heart and trachea on X-ray
  • the bronchus of the affected side would be absent on endoscopic view
20
Q

What is pulmonary hypoplasia?

What is it often associated with?

A
  • this occurs when all the components of the lung are present, but incompletely developed
  • the severity of hypoplasia determines the degree of respiratory compromise
  • there is an abnormally low number or size of bronchopulmonary segments or alveoli
  • it is often found in association with a congenital diaphragmatic hernia (CDH)
21
Q
A
22
Q

How can maturation of the lungs be divided into 4 periods?

A
  1. pseudoglandular
  2. canalicular
  3. saccular / terminal sac
  4. alveolar
23
Q

When does the pseudoglandular period take place?

What happens during this period?

A
  • occurs between weeks 5-17
  • branching of the respiratory tree occurs to form terminal bronchioles
  • this begins as the bronchial tree develops as solid tubes, which bud to form bronchi, bronchioles and terminal bronchioles
24
Q

Could a foetus born during the pseudoglandular period survive?

A
  • No, a foetus could not survive as respiration is not possible at this stage
  • no respiratory bronchioles or alveoli are present during this period
25
Q

When does the canalicular phase take place?

What are the major changes that happen to the developing lungs during this period?

A
  • the canalicular phase occurs between weeks 16-25
  • the terminal bronchioles divide to form respiratory bronchioles
  • each respiratory bronchiole divides into 3-6 alveolar ducts
  • the ducts end in terminal sacs (primitive alveoli) that are surrounded by flat alveolar cells in close contact with neighbouring capillaries
26
Q

What happens to the vascular supply during the canalicular period?

Would a foetus born during this period be able to survive?

A
  • mesodermal tissue becomes highly vascularised during this period
  • respiration is possible towards the end of the canalicular period as sufficient numbers of terminal sacs have developed at the end of respiratory bronchioeles
  • there are also sufficient numbers of capillaries to allow for adequate gas exchange
27
Q

When does the terminal sac period take place?

What happens during this period?

A
  • this occurs from week 26 - birth
  • the number of terminal sacs (primitive alveoli) steadily increases
  • type I alveolar epithelial cells lining the terminal sacs become thinner, allowing capillaries to protrude into the alveolar sacs
  • intimate contact between epithelial and endothelial cells forms the blood-air barrier
28
Q

How does the epithelium change during the terminal sac period?

A
  • it differentiates to give rise to type I and type II pneumocytes

Type I pneumocytes:

  • these are the thinned cells that line the alveolar sacs
  • gas exchange takes place across these cells

Type II pneumocytes:

  • these produce surfactant, which is needed to lower surface tension at the air-alveolar interface
  • reducing surface tension facilitates inflation
29
Q

Can a foetus born during the terminal sac period survive?

A
  • a foetus born prematurely (24 weeks) can survive in intensive care but may suffer from respiratory distress syndrome
  • mature alveoli are not present before birth
30
Q

When does the alveolar period take place?

What happens during this period?

A
  • the alveolar period takes place between 36 weeks and 8 years
  • development of the lungs after birth is mainly due to an increase in the number of respiratory bronchioles and alveoli
  • 95% of mature alveoli do not develop until after birth
31
Q

When do foetal breathing movements start?

Why is this important?

A
  • foetal breathing movements begin in utero to allow for removal of amniotic fluid
  • these movements stimulate lung development and condition respiratory muscles
32
Q

What happens when respiration begins at birth?

A
  • any remaining lung fluid is rapidly resorbed by blood / lymph capillaries
  • when fluid is resorbed from alveolar sacs, a thin layer of surfactant remains deposited as a thin phospholipid coat on alveolar cell membranes
  • when air enters the lungs at the first breath, surfactant prevents collapse of the alveoli during expiration and allows for respiration
33
Q

How does lung development influence survival of a premature infant?

A
  • the state of development of the lungs is a prime factor in determining prognosis when a child is born prematurely
  • if they are born after 26 weeks, chance of survival is good as surfactant is produced in sufficient quantities
    • this is during the terminal sac period
  • if they are born prior to 26 weeks, the chance of survival is poor as insufficient quantities of surfactant have been produced
34
Q

What happens in infant respiratory distress syndrome (RDS) at birth?

How can this develop into a chronic condition?

A
  • laboured breathing threatens the infant with immediate asphyxiation
  • there is an increased rate of breathing
  • mechanical ventilation is needed to support breathing
  • damage to the alveolar lining leads to fluid and serum proteins leaking into the alveolus
  • continued injury can lead to detachment of the alveolar lining
  • chronic lung injury in preterm infants can cause bronchopulmonary dysplasia
35
Q

What happens in bronchopulmonary dysplasia?

A
  • it is a chronic lung disease in which premature infants, usually those who were treated with supplemental oxygen, require long-term oxygen therapy
  • the alveoli that are present tend not to be mature enough to function normally
36
Q

What are the treatments available for infant respiratory distress syndrome?

A
  • glucocorticoid treatment accelerates foetal lung development and surfactant production
  • surfactant therapy can be performed with natural or artificial surfactants

it is more effective when surfactant A and B proteins are used

37
Q

What is surfactant protein B deficiency disease?

What is the prognosis?

A
  • this is an autosomal recessive condition
  • it leads to lethal respiratory failure within the first year of life despire mechanical ventilation and surfactant therapy