Embryology of the Lungs Flashcards
During what week does the respiratory diverticulum appear?
- the respiratory diverticulum appears as an outpouching from the ventral wall of the foregut during week 4
What substance and transcription factor are important in determining the location and appearance of the lung bud (respiratory diverticulum)?
- appearance and location of the lung bud are dependent on an increase in retinoic acid produced by adjacent mesoderm
- an increase in RA upregulates TBX4 expressed in the endoderm of the gut tube at the site of the lung bud
- TBX4 induces formation of the lung bud and continued growth and differentiation of the lungs
From which germ cell layer are the lungs developed from?
- the epithelial lining of the larynx, trachea, bronchi and lungs is of endodermal origin
- the cartilagenous, muscular and connective tissue components of the trachea and lungs are derived from splanchnic mesoderm surrounding the foregut
On what day does the lung bud appear?
How does it grow and what separates it from the foregut?
- it appears on day 22 and is initially in open communication with the foregut
- as it grows ventrocaudally, 2 longitudinal tracheo-oesophageal ridges form to separate the respiratory diverticulum from the foregut
- the lung bud remains in communication with the laryngeal inlet
What happens to the tracheosophageal ridges after they have formed?
- the tracheoesophageal ridges fuse to form the tracheoesophageal septum
- this divides the foregut into a dorsal portion (oesophagus) and ventral portion (trachea and lung buds)
- the respiratory diverticulum then divides to form 2 lung buds
What is a tracheosophageal fistula and why does it occur?
- TOFs result from incomplete division of the foregut into oesophageal and respiratory portions
- abnormalities in partitioning of the oesophagus and trachea by the tracheoesophageal septum results in oesophageal atresia +/- TOFs
- a fistula describes an abnormal connection
What are TOFs associated with in 90% of cases?
closed or absent oesophageal atresia
- the upper portion of the oesophagus ends in a blind pouch
- the lower segment of the oesophagus forms a fistula with the trachea
What 2 variations of TOFs each account for 4% of cases?
- isolated oesophageal atresia where there is no formation of a fistula
- H-type TOF without oesophageal atresia
What is the most common complication of TOF with oesophageal atresia?
- the upper oesophagus ends rapidly and lower oesophagus forms a fistula with the trachea
- the abdomen rapidly distends as the stomach fills with air
- it can also cause aspiration pneumonia as milk can enter the trachea from the blind-ended sac
What is the clinical consequence of a H-type TOF?
- this can lead to milk being “driven” into the respiratory system
What other congenital abnormalities are TOFs usually associated with?
- they are most commonly associated with cardiac defects (1/3 of cases)
- they are part of the VACTERL association - a collection of defects with unknown causation but occur more frequently than predicted by chance alone
What is the first stage in formation of the lungs?
What happens during week 5 of development?
- during partitioning of the oesophagus and the lung bud, right and left bronchial buds form
- further growth and differentiation at the start of week 5 leads to enlargement of the bronchial buds to form right and left main bronchi
- the right main bronchus forms 3 secondary bronchi
- the left main bronchus forms 2 secondary bronchi
How do the secondary bronchi further divide during week 6 of development?
- further branching results in the formation of tertiary bronchi, which will each supply a bronchopulmonary segment
- there are 10 tertiary bronchi on the right and 8 on the left
Following the formation of tertiary bronchi, what further divisions occur?
How is this branching regulated?
- branching continues to form terminal bronchioles by week 16 and respiratory bronchioles by week 26
- the first alveoli develop in week 36
- branching is regulated in part by the interaction of the epithelium (derived from the foregut) with the overlying visceral mesoderm
- the visceral mesoderm forms the cartilage, smooth muscle, connective tissue and capillaries
What forms the 2 different types of pleura?
- visceral mesoderm forms the visceral pleura
- parietal mesoderm forms the parietal pleura