ELISA: principle, steps, applications.

Question 1

ELISA

Answer 1

test used to detect if certain substances like antibodies, proteins, hormones, or other biomolecules are in a sample.

Question 2

Elisa type

Answer 2

1. Qualitative ELISA result = positive or negative
2. Quantitative ELISA = optical density of sample is compared to a standard
3. Indirect
4. Competitive
5. Sandwich

Question 3

ELISA steps

Answer 3

1 first antibody is bound to the plate wall
2 antigen is added
3 incubation
4 wash the plate 3 times
5 second antibody is added
6 incubation
7 stop the reaction

Question 4

ELISA application:

Answer 4

1. Food allergen
2. Biomarker for various pathologies
3. Toxicology: drug Hormones: stress, pregnancy
4. Virus: HIV SARS COVID