Elisa lab Flashcards
ELISA
Technique used in a wide variety of applications
Medicine – Measure amounts of disease-related proteins in serume.g. Allergy Tests, Prostate Cancer, HIV test
Veterinary – Spread of disease (e.g. Bird Flu)
Pharmaceuticals –Identify biomarkers in non-infectious disease (e.g. atherosclerosis)
Environmental – Measure of environmental pollutants in water
Lifestyle/Home use kits – Pregnancy Test, Chlamydia Test
Purpose and aims of ELISA Laboratory Experiment
Follow the an immunisation schedule against a foreign protein (albumin), monitor the amount (titre) of specific antibody in the blood at defined time points after the organism has been immunized/vaccinated
Aims:
Monitor changes in the titre of antisera during an immunisation schedule
Determine whether the antisera, taken after the third immunisation, is specific to human albumin
Whats albumin
Albumin is a protein made by your liver. Albumin helps keep fluid in your bloodstream so it doesn’t leak into other tissues. It is also carries various substances throughout your body, including hormones, vitamins, and enzymes
PBS
Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research.It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH.
