Hydrophobic interaction chromatography (HIC)

Question 1

Ligand:

Answer 1

: The molecule bonded to the resin to effect a separation (e.g. C18)

Question 2

Spacer arm

Answer 2

Spacer arm: usually an aliphatic chain with one end attached to the resin and the other end attached to the ligand. It allows the ligand to distance itself from the surface of the resin and provide a better interaction with the target molecule