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Immune Microbiology > ELISA > Flashcards

ELISA Flashcards

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1
Q

What does ELISA stand for?

A

Enzyme linked immuno-sorbant assay

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2
Q

What is the purpose of an ELISA test?

A

To detect the presence of antibodies or antigens in a sample

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3
Q

True or False: ELISA is a commonly used diagnostic test in medicine.

A

True

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4
Q

Fill in the blank: ELISA is a ____________-based assay technique.

A

enzyme

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5
Q

What type of enzyme is commonly used in ELISA tests for detection purposes?

A

horseradish peroxidase

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6
Q

What is the color change mechanism used in ELISA tests?

A

substrate conversion by the enzyme

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7
Q

What are the two main types of ELISA tests?

A

indirect ELISA and sandwich ELISA

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8
Q

What is the key difference between indirect ELISA and sandwich ELISA?

A

The way the antigen is detected

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9
Q

True or False: ELISA tests are quantitative in nature.

A

True

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10
Q

What is the typical incubation time for an ELISA test?

A

1-2 hours

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11
Q

What is the purpose of adding a stop solution in an ELISA test?

A

To halt the enzyme reaction

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12
Q

Fill in the blank: The signal in ELISA tests is typically __________ to the amount of antigen present.

A

proportional

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13
Q

What is the advantage of using ELISA tests in terms of sensitivity?

A

High sensitivity

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14
Q

Which type of ELISA test is more commonly used for detecting antibodies?

A

Indirect ELISA

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15
Q

What is the role of the substrate in an ELISA test?

A

To produce a color change for detection

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16
Q

True or False: ELISA tests can be automated for high-throughput screening.

A

True

17
Q

What is the main advantage of using ELISA tests over other immunoassay techniques?

A

High specificity

18
Q

What does the optical density in an ELISA test indicate?

A

The amount of antigen present

19
Q

Fill in the blank: ELISA is a widely used technique in __________ research.

A

biomedical

20
Q

What is the main limitation of ELISA tests?

A

Cross-reactivity with similar antigens

21
Q

What are some common applications of ELISA tests?

A

Diagnosis of infectious diseases, detection of allergens, monitoring autoimmune diseases

22
Q

True or False: ELISA tests have revolutionized the field of diagnostics.

A

True

Immune Microbiology (5 decks)

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