electrophoresis Flashcards
1
Q
what determines whether an AA is acidic/basic
A
r group
ends in NH2 - base
ends in COOH - acidic
H3N or COO- neutral
2
Q
def of electrophoresis
A
migration of macromolecules in an electric field
3
Q
what is migration based
A
size, shape, charge, current, resistance
4
Q
what mobile phase is used in horizontal electrophoresis
A
agarose gel
5
Q
what mobile phase is used in vertical
A
polyacrylamide
6
Q
why does the slab of gel get immersed in buffer
A
buffer provides ions, to carry current & maintain pH, maintains heat/medium for heat dissipation
7
Q
what is (poly)acrylamide
A
acrylamide polymerised in presence of APS and TEMED, smaller pores than agarose
8
Q
which kind is most commonly used for protein electrophoresis
A
vertical
