Electrophoresis Flashcards
Ampholyte
Molecule that’s positively or negatively charged based on pH of the solution
Anode
Positive electrode
Attracts Anions
Cathode
Negative Electrode
Attracts Cations
Densitometry
Measurement using fraction separated on a gel
Electrophoresis
Migration of charged particles in a liquid medium of an electrical field
What happens when molecules move at different rates?
Separation
What can affect the speed a molecule moves at?
Charge
Size
Viscosity
Voltage
What are commonly used support mediums?
Cellulose Acetate
Agarose Gel
What is Integration?
A tracing or graph is produced
percentage of the total is printed
Densitometer
What is the procedure of Electrophoresis?
Separation
Support material removes excess; then added
Sample is added to support material
Electrophoresis is conducted under constant voltage
What do we need to do to see the results of Electrophoresis?
Stain for detection
What are the particles of interest in the lab?
Proteins
Serum
Urine
Cerebrospinal Fluid (CSF)
What are the two types of Electrophoresis?
Zone and Capillary
What distinguishes Zone Electrophoresis?
Divided into bands (fraction/sections)
Classified by the type of specimen and materials used
What distinguishes Capillary Electrophoresis?
In a small tube
Enhanced separation efficiency
Can be less than 1 minute
More expensive
