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Analytical Techniques and Instrumentation > Chromatography > Flashcards

Chromatography Flashcards

1
Q

Chromatography

A

Separates mixtures by difference in physical interactions

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2
Q

What two phases are present in Chromatography?

A

Stationary and Moving

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3
Q

What does the Mobile Phase carry a sample through?

A

Bed
Layer
Column

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4
Q

What are the two types of Chromatography?

A

Planar
Column

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5
Q

Planar Chromatography

A

Stationary phase is coated on a sheet of paper or bound to a solid surface

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6
Q

Column Chromatography

A

Stationary phase is packed into a tube or bonded to the inner surface of a tube and support particles

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7
Q

What is the technique called when the Mobile Phase involves a gas or a liquid?

A

Gas or Liquid Chromatography

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8
Q

What is the technique when we combine gas and liquid chromatography?

A

Gas Chromatography-Mass Spectrometry (GCMS)
Liquid Chromatography-Mass Spectrometry (LCMS)

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9
Q

What are the procesess used to separate solutes?

A

Ion-Exchange
Partition
Adsorption
Size-Exclusion
Affinity Mechanisms

I Party All Season Anyway

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10
Q

Ion-Exchange Chromatography

A

Exchange of ions during stationary
Ions of opposite charge during mobile

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11
Q

What are the negative functional groups in Cation-Exchange particles?

A

Carboxyl Ions
Phosphate Ions
Sulfonate Ions

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12
Q

What separations does Ion-Exchange include?

A

Amino Acids
Oligonucleotides
Glycated Hemoglobin
Hemoglobin Variants

Always Order Good Ham

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13
Q

Partition Chromatography

A

Differential between two immiscible liquids
Based on differences in solubility

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14
Q

What is Partition Chromatography classified as?

A

Gas-liquid Chromatography (GLC)
Liquid-liquid Chromatography (LLC)

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15
Q

Adsorption Chromatography

A

Differential adsorption of solutes in stationary phase

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16
Q

What are the two types of Adsorbents?

A

Nonpolar and Polar

17
Q

What are the Polar Adsorbents used in Liquid Chromatography?

A

Silica and Alumina

18
Q

Size-Exclusion Chromatography

A

Separates based on size and shape
AKA Gel-Filtration or Molecular Sieve

19
Q

What is Size-Exclusion Chromatography used for?

A

Separate large molecules such as Proteins/nucleic acids from small molecules like salts/oligonucleotides

20
Q

Affinity Chromatography

A

Binds to the support bound ligand

21
Q

What creates separation in Affinity Chromatography?

A

Antibody binding to antigen

22
Q
A

Analytical Techniques and Instrumentation (6 decks)

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