Electron Transport Chain Flashcards

1
Q

Where are the electron transport chains located?

A

they are embedded in the inner membrane of the mitochondria

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2
Q

Which is more acidic? The cytosol or the mitochondrial matrix?

A

the cytosol is more acidic. Mitochondrial matrix is more alkaline.

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3
Q

Where does oxidation of NADH take place?

A

Aerobic oxidation of NADH takes place in the mitochondria

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4
Q

Transportation of NADH and ATP

A

1) need to get much of the cytosolic NADH into the mitochondria and NAD+ back out
2) Need to get much of the cytosolic ADP and Pi into the mitochondria and the ATP out

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5
Q

How can I make oxa cross the membrane?

A

Make asp

  glu     a-KG
    l\_\_\_\_l oxa -------------> asp
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6
Q

In what organs is the malate-aspartate shuttle dominant?

A

in the liver, kidney, and heart

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7
Q

Where is the glycerol-3-phosphate shuttle dominant?

A

in skeletal muscle and brain

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8
Q

Which shuttle allows for cells to get more ATP? glycerol-3-P, or mal-asp shuttle?

A

mal-asp shuttle = more ATP for cells to use

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9
Q

How are ATP, ADP, and Pi transported across membrane?

A

mitochondrial electrical gradient used to transport ATP, ADP, and Pi as seem in diagram (draw diagram, ya goof)

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10
Q

Which large complexes are used in e- transport chain?

A

I, III, and IV (and 2 small electron carriers)

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11
Q

In the mitochondrial electron transport chain electrons are transferred through a series of redox reaction from _________ to _______-

A

NADH to O2

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12
Q

What is cytochrome C?

A
  • small protein
  • diffuses rapidly
  • located in the intermembrane space
  • carries electrons from Complex III to Complex IV
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13
Q

What is Q in electron transport chain?

A
  • ubiquinone
  • small lipid redox partner that moves inside the inner mitochondrial membrane
  • small. diffuses rapidly
  • between complexes I and III
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14
Q

How many electrons are removed in the conversion of mitochondrial NADH to NAD+? Where do they go?

A

2 electrons removed.

they are given to Q and then to Complex III

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15
Q

Where do the electrons go from complex III?

A

to cytochrome C, to complex IV, and then used to reduce O2 to H2O

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16
Q

In complex I, which step has the largest stndard free energy change?

A

Not sure. Ask

17
Q

According to the chemiosmotic theory, what do the protons re-entering the mito do?

A

The energy used for the H+ to re-enter cell generate ATP

18
Q

What is the role of al F1Fo-ATPase?

A

to phosphorylate ADP to ATp

19
Q

How many H+’s need to cross to make one ATP?

A

about 3

20
Q

Which complex is the rate determining step?

A

Complex IV

21
Q

Brown adipose

A

has a transporter to allow H+ back into the mito without coupling it to ATP synthesis.
Energy becomes HEAT, used to warm organism