Electron Transport Chain

Question 1

How much energy is produced per glucose?

Answer 1

2 ATP, 10 NADH (2 cytosolic and 8 mitochondrial), 2 FADH2 and 2 GTP produced per glucose -> 30-32 ATP in total

Per glucose the Krebs cycle happens twice

Question 2

Protons required per ATP

Answer 2

If in yeast 10 protons are required to turn the ATPsynthase through one complete cycle to synthesise 3 ATP - also the phosphate symporter pumps in 3 protons with 3 Pi and to synthesise those 3 ATP
(10+3)/3 = 4.33 protons per ATP

In mammals only 8 protons are required to turn the ATP synthase through one complete cycle
(8+3)/3 = 3.66 protons per ATP

So estimated 4 protons are required synthesise 1 ATP

If 4 protons are required synthesise 1 ATP
How many ATP synthesised per NADH and FADH2

• 10 protons are pumped into the IMS per NADH
  10/4 = 2.5 ATP per NADH
• 6 protons are pumped into the IMS per succinate (FADH2)
  6/4 = 1.5 per FADH2