Electron transport chain

Question 1

Basal matabolic rate

Answer 1

measure of total daily energy expenditure by body at rest

Question 2

How to measure efficiency of oxidative phosphorylation or what is P:O ratio?

Answer 2

The efficiency of oxidative phosphorylation can be measured by dividing the amount of phosphate incorporated into ADP by the amount of atomic oxygen reduced - that is, the P : O ratio

Question 3

FAD and FMN contains which vitamin

Answer 3

contains water soluble vitamin Riboflavin

Question 4

Redox shuttles and their location

Answer 4

Glycerol 3 phosphate shuttle : Skeletal muscle
Malate - aspartate shuttle : Heart and liver

Question 5

Chemiosmotic hypothesis of ATP synthesis

Answer 5

synthesis of ATP in mitochondia uses energy from H gradient created across mitochondrial membrane by oxidation of NADH and FADH2

Question 6

Which cofactor is required to transfer electron from complex IV to oxygen

Answer 6

Copper

Question 7

Inhibitors of ETC

Answer 7

Rotenone - inhibits complex I - revert by Tx with succinate
Antimycin - inhibit complex III - revert by ascorbic acid
Cyanide and carbon monoxide - inhibit complex IV, affect transport of oxygen and ATP synthesis. Revert by O2 and vitamin B12
Oligomycin - Inhibit ATP synthase. Increase proton gradient in IMM