effect of temperature + q10 Flashcards
how does temp impact enzymes
molecules move faster as more KE so collide more and greater force so more chance successful collision
more ESC so more EPC
faster rate
effect if temp over optimum
mols vibrate
weakens bonds
tertiary structure destroyed
loss 3d shape
active site not comp to substrate no ESC
denatured
rate decreases
irreversible
what is optimum temp
40 to 50 degrees
plant = 20degrees
what is Vmax
temp give opt rate
what is initial rate reaction
rate largest at start as conc substrate higher
more chance sub to active site
all active site occupied
burst phase
what is Q10
measure of rate of change when inc temp by 10degrees
how to calc q10
rate at x+10degrees/ rate a x degrees
normally 2
interpret q10
1= not temp sensitive
larger q10 faster rate rises (steeper curve graph)
