ECM

Question 1

What is ECM?

Answer 1

noncellular component in all tissues and organs. provides physical scaffolding, structure support and cues for morphogenesis, differentiation and homeostasis

Question 2

Interstitial is composed of?

Answer 2

Fibrillar collagens (type I,II,III)
Elastin
Fibronnectin
Hyaluronic acid
Proteoglycans

Question 3

Basement membrane is composed of?

Answer 3

Type IV collagen
Laminin
Heparin Sulfate Proteoglycans

Question 4

Collagen?

Answer 4

Most abundant ECM protein for structure and cell adhesion, various types serves various purposes.

Question 5

Fibronnectin?

Answer 5

Dimer containing domains for cell binding ( integrin binding sequence is the Arg-Gly-Asp, RGD sequence), heparin and fibrin also binds.

Mediates cell attachment and function.

Question 6

Laminin?

Answer 6

Trimeric crosslinked polypeptide found in basement membrane. Important for self assembly of cell populations and tissue organisations

Question 7

Elastin?

Answer 7

Highly branched hydrophobic protein, elastic, present in skin, lungs, blood vessels

Question 8

Proteoglycans?

Answer 8

Fibrillar structure, immobilisation of growth factors, cellular signal, cell-matrix adhesion

Question 9

Glycosaminoglycans?

Answer 9

unbranched polysaccharide chains. Helps keep ECM hydrated

Question 10

Name ECM growth factors

Answer 10

Glycoproteins, EGF, IGF, VEGF, FGF

Question 11

Matrix degrading enzymes?

Answer 11

Glycosidases, Proteinases, Cysteine proteinases, Matrix metalloproteinases.

Question 12

ECM biomaterials

Answer 12

Collagen, Fibrin, Matrigel. These scaffolds can be mechanically weak though