Biofabrication as an evolving field

Question 1

What are traditional biomaterials?

Answer 1

Inorganics (like metals)

Organics

Question 2

What is histochemical staining?

Answer 2

A staining method for detecting proteins, carbohydrates, lipids in cells.

Question 3

immunofluorescence

Answer 3

Staining of biomolecular targets in cells using antibodies carrying fluorophores for binding the desired antigens, and is visualized by fluorescence microscopy

Question 4

What is optogenetics?

Answer 4

engineered light-sensitive receptors “opsins” which can excite or inhibit neuronal signaling in animals

Question 5

Essentials in biofab:

Cellular component?

Answer 5

Cell source - stem cells, cell lines, numbers and combinations

Question 6

Biomaterials?

Answer 6

Proteins, polymers, physical chemical properties, biochemical signals

Question 7

Fabrication?

Answer 7

Modality, compatibility, cell stress

Question 8

maintenance/maturation

Answer 8

Tissue culture media, bioreactors, physical conditions

Question 9

mammalian cell size?

Answer 9

10-100 µm, usually around 80µm

Question 10

What is bioprinting?

Answer 10

3D printing with cells can be inkjet, contact, microextrusion, or laser-assisted bioprinting

Question 11

Name different electrospinning techniques

Answer 11

melt electrospinning, 3D electrowriting, drum electrospinning, wet spinning