ECFV Flashcards
How do KD play a central role in this process by regulating the volume of ECFV?
• plasma volume via changes in ECFV and RBC volume via erythropoietin.
How is the ratio of the two subcompartments of ECF (i.e. the interstitial fluid and blood plasma) determined by the Starling forces?
- If blood volume is high, capillary pressure increases and fluid is filtered out from the plasma into the interstitium.
- If blood volume declines, fluid is driven into the blood from the interstitium by the negative oncotic pressure.
What is the clinical relevance of the KD and blood pressure?
- The kidneys play an overriding role in regulating BP.
* Treatment of hypertension is directed at renal handling of NaCl.
What are the three routes of Na loss?
- The typical daily intake of Na on a Western diet is ~120 mEq.
- Na (~90%) is excreted via the kidneys, ~7% by the feces and ~3% through the skin.
Do the KD have a role in Na conservation of the GI and skin?
- With low Na intake, in addition to renal Na conservation, Na loss via the GI tract and the skin must be also curtailed.
- Interestingly, the signals that control these latter routes of Na loss, also originate from the kidney.
What governs salt appetite?
- Na appetite, which is analogous to the mechanism of thirst.
- The signals that govern salt appetite also emanate from the kidney.
Although regulation of ECFV is critical for the regulation of blood pressure, it is not ECFV as a whole that is sensed and regulated.
• All receptors that govern Na balance, and thus ECFV, reside in the circulatory system.
• They fall into two main categories
o high-pressure receptors
o low-pressure receptors.
Where do the dominant High Pressure Receptors reside?
- The dominant high-pressure receptors are in the afferent arterioles in the kidney.
- The high-pressure receptors in the carotid sinus and aortic arch play a minor role.
Why do high pressure receptors in the carotid sinus and aortic arch play only a minor role?
• The role of the carotid sinus and aortic arch receptors is the short-term control of blood pressure, and to protect the brain from ischemia.
• baroreceptors in large arteries adapt to a continuous increase in arterial pressure (i.e. hypertension) within a few days.
o The essence of this adaptation is the thickening of arteries by the proliferation of smooth muscle cells and the incorporation of more connective tissue. Due to this anatomical restructuring, the increase in wall tension (which is the stimulus for the baroreceptors) is transient.
Why are the renal afferent arterioles the dominant high-pressure receptors?
- Vessel restructuring does not occur in the afferent arterioles as they are composed of a single layer of muscle cells, even in hypertensive states.
- The renin-producing granular cells at this site lack contractile fibers, and therefore always experience a stretch that is proportional to the actual blood pressure.
Why do we need low pressure receptors? Where are they located?
- Low-pressure receptors are important for the intermediate-term (hours) regulation of blood pressure.
- These receptors reside in the cardiac atria, the vena cava and large pulmonary vessels.
Why do low-pressure receptors show the greatest response to ECF volume changes?
• Since these structures are much more distensible and most of the blood resides at the low-pressure end of the circulation, low-pressure receptors show the greatest response to changes in blood volume.
Why is a dual regulation by high- and low-pressure receptors is necessary?
What is “Effective Circulating Volume” ?
• A dual regulation by high- and low-pressure receptors is necessary, because blood pressure is not only dependent on the total volume of blood but also on its the distribution within the vasculature.
o In upright position a significant portion of the blood pools in the lower extremities and therefore not available for perfusing tissues.
o The total blood volume minus this un-measurable amount of pooled blood that circulates sluggishly is termed “effective circulating volume”.
Thought Question. Explain how low-pressure receptors effect Na excretion when a person is immersed in a pool of water? Why aren’t high-pressure receptors involved?
- This is a case of “effective circulating volume”
- outside hydrostatic pressure compresses the extremity blood vessels thereby increasing the amount of blood in the thorax.
- Since water immersion results in a substantial increase in renal Na excretion without a change in arterial pressure, the natriuresis is mediated solely by stimulation of low-pressure receptors in the thorax.
Thought Question. Why would heart failure stimulate renal Na retention?
- Congestive heart failure is associated with avid renal Na reabsorption in spite of a significant increase in venous pressure.
- During prolonged increase in venous pressure, low-pressure receptors are reset by a mechanism similar to that described above for high-pressure receptors and therefore do not signal to excrete Na.
- At the same time, as a consequence of the failing heart, renal arteriolar pressure is reduced, which generates signals that increase Na reabsorption (and edema).
What are the main mechanisms for Na reabsorption?
- Renin-Angiotensin-Aldosterone System (RAAS)
* And activation of the renal sympathetic nerves.
What is the main inhibitor of Na reabsorption?
• Atrial Natriuretic Peptide (ANP).
With changes in effective circulating volume, the kidney makes appropriate adjustments in Na excretion. Low- and high-pressure receptors regulate renal Na reabsorption by altering the balance between natriuretic and anti-natriuretic mechanisms.
What is the sequence of the RAAS system? Where is each enzyme/hormone produced?
- The RAAS dwarfs all other Na balance mechanisms.
- renin, is a proteolytic enzyme produced by the granular cells of the afferent arterioles.
- renin cleaves angiotensinogen, a circulating protein produced by the liver to generate Angiotensin I (AI)
- AI itself is biologically inactive.
- It is converted into the biologically active form, Angiotensin II (AII), by further proteolysis catalyzed by Angiotensin Converting Enzyme or ACE.
- ACE is an ectoenzyme that is expressed on the surface of most endothelial cells.
How does AII facilitates Na retention?
• AII facilitates Na retention by several synergistic mechanisms that include both renal and extrarenal actions.
What are the main renal effects of AII?
• AII directly stimulates Na reabsorption in the proximal tubule by increasing the activity of the apical Na/H exchanger.
• AII also enhances renal Na conservation by affecting several aspects of renal hemodynamics:
o a) It lowers the set point and heightens the sensitivity of the TGF, and thus an increase in NaCl load delivered to the macula densa will trigger a more robust decrease in GFR.
o b) By constricting the efferent arterioles, AII tilts the peritubular Starling forces in favor of reabsorption by the proximal tubule (decreased hydrostatic & increased oncotic pressure).
• Students are occasionally confused by the consequences of AII-mediated efferent constriction, since such an effect by itself would tend to increase GFR, which seems counterproductive.
• Keep in mind that AII levels increase only when blood pressure declines, and thus, this effect helps to preserve GFR rather than increasing it.
o c) AII also reduces medullary blood flow, which in turn enhances urinary concentrating ability and increases Na+ reabsorption in the thin ascending limb of the loop of Henle.
What is the main extrarenal (and most important) effect of AII? What are secondary effects?
• The main effect is stimulation of aldosterone
• Secondary: AII is a very potent vasoconstrictor because its direct effects on vascular smooth muscle are amplified by several indirect mechanisms.
o These include increased sympathetic activity, diminished vagal tone and baroreflex sensitivity, enhanced norepinephrine release and reduced reuptake at sympathetic terminals
• Secondary: AII stimulates thirst, and (to a lesser degree) Na appetite.