E3 Intro Proteins

Question 1

Proteins lacking in serum

Answer 1

5 8 fibrinogen

Question 2

Serum vs plasma

Answer 2

Serum is what remains after blood clots (red top)
Plasma is the fluid remaining when blood is prevented from clotting

Horses and exotics hepranized (prevents clotting) plasma used for serum biochem because naturally takes so long to clot

Question 3

Three main plasma proteins

Answer 3

Albumin
Globulins
Fibrinogen
(+other)

Question 4

Where are most plasma proteins sxn?

Answer 4

Liver

Question 5

Where are immunoglobulins sxn?

Answer 5

Lymphoid organs

Question 6

Plasma proteins in greyhounds

Answer 6

Usually lower than other dogs

own reference intervals

Question 7

Major protein contributing to colloid osmotic pressure (COP)

Answer 7

Albumin (80%)

Globulins 20%

Question 8

Protein measurement methods

Answer 8

Refractometer (Physical)
Spectrophotometer (biochemical)
Electrophoresis (Fractionation)

Question 9

Causes of increased fibrinogen

Answer 9

Active inflammation (esp LA)
Physiologic stress

Question 10

Causes of decreased fibrinogen

Answer 10

DIC

Other: Snake bites/liver failure

Question 11

Best way to analyze exotic species protein

Answer 11

Electrophoresis

Question 12

Protein and RBC concentration with external hemmorag

Answer 12

Acute may be normal then fluid rapidly replaced causes

HYPOProteinemia and anemia

Question 13

Protein and RBC concentration with dehydration

Answer 13

Relative hyperproteinemia and erythrocytosis

Question 14

General Mechanism of hypoproteinemia

Answer 14

Decreased production
Increased loss
Sequestration
Dilution

Question 15

General mechanisms of Hyperproteinemia

Answer 15

Hemoconcentration

Hyperglobulinemia