E&M, Cytologic Techniques, Tissue Processing Flashcards
(from the Greek mikros, meaning “small”, and temnein, meaning “to cut”) is a tool used to cut extremely thin slices of material, known as sections.
Microtome
The rotary microtome is the most used microtome in the Histopathology laboratory.
_____ is an instrument that is used to analyze and process samples by fixing, staining, dehydrating, or decalcifying them. It removes excess water from the tissue for it to be able to coat in melted paraffin wax.
Automatic tissue processor
_____ is designed to ensure that reagent solution spread evenly over the slide, and remain in place during the ensuing incubation period.
Automatic slide staining machine
It is designed for high-precision temperature control of embedding mediums like paraffin wax.
Paraffin oven
_____ is an essential step in the histology process, which allows the paraffin ribbon to smooth out and stick to the glass slide when placed.
Water bath
_____ is designed to assist with the handling of paraffin wax samples in histology and pathology laboratories. It is essentially a hot distilled water floating out bath that allows for the meticulous manipulation and location of sections onto glass slides.
Paraffin embedding wax bath
_____ is a rotor-driven instrument designed to separate liquid samples at high rates of speed.
Centrifuge
_____ is an appliance for removing excess paraffin from tissue cassettes
Paraffin block trimmer
_____ is designed to rapidly dry slides by blowing warm air over the surface of the slides.
Slide dryer
_____ is used to visualize very minute objects such as cells, microorganisms, giving a contrasting image, that is magnified.
Microscope
95% alcohol
-Pap smear staining-
Used in fixation (15-30 min)
80% alcohol
-Pap smear staining-
Used in hydration (2 min)
60% alcohol
-Pap smear staining-
Used in hydration (2 min)
1st Coplin jar of distilled water
-Pap smear staining-
Used in hydration (5 dips)
2nd Coplin jar of distilled water
-Pap smear staining-
Used in hydration (5 dips)
Hematoxylin stain
-Pap smear staining-
1st nuclear stain (3 min)
Running water
-Pap smear staining-
Gentle washing (30 sec)
Scott’s tap water
-Pap smear staining-
Blueing agent, pH=8.02 (3 min)
Distilled water
-Pap smear staining-
Dehydration (3 min)
60% alcohol
-Pap smear staining-
Dehydration (2 min)
80% alcohol
-Pap smear staining-
Dehydration (2 min)
1st Coplin jar of 95% alcohol
-Pap smear staining-
Dehydration (2 min)
Orange G stain
-Pap smear staining-
1st counterstain (3 min)
1st Coplin jar of 95% alcohol
-Pap smear staining-
(2 min)