E. Coli Flashcards
Differentiate between commensal E. coli and enteropathogenic E. coli.
Commensal E. coli: part of normal intestinal microflora (0.1%)
- -Make vitamin K and some B vitamins
- -Protects against other pathogens at mucosa
E. coli intestinal pathogens are STRAINS that have acquired VIRULENCE FACTORS through horizontal gene transfer
Understand the processes involved in the “evolution” of a pathogen.
Evolve by HORIZONTAL GENE TRANSFER via..
- -Phage
- -Plasmids
- -Transposons
- -Gene loss
List the toxins secreted by each of the six E. coli strains discussed.
EHEC: STX, hemolysins ETEC: ST, LT EPEC: no toxins EAEC: hemolysins, EAST EIEC: no toxins DAEC: no toxins
List the shiga toxin-producing E. coli (1) and the diarrheagenic E. coli. (4)
Shiga toxin-producing EC (STEC): EnteroHemorrhagic EC (EHEC)
Diarrheagenic: (PATI-D) EnteroPathogenic EC (EPEC) EnteroAggregative EC (EAEC) EnteroToxigenic EC (ETEC) EnteroInvasive EC (EIEC) Diffuse Adhering EC (DAEC)
What is the most common EHEC serogroup?
O157:H7
How is EHEC spread? What is its infectious dose?
Foods: --Undercooked/contaminated ground beef --Leafy vegetables --Unpasteurized apple cider/juice --Raw-milk dairy products Animals (house pets, petting zoos) Person-to-person contact
Infectious dose: very low, 100
What are the clinical symptoms of EHEC?
Abdominal pain
Bloody diarrhea
**Hemolytic uremic syndrome - can -> renal failure
What are the symptoms of hemolytic uremic syndrome? (4) What causes it? How can it be treated?
Destruction of RBCs, damage to lining of vessel walls due to apoptosis/necrosis; hemolytic anemia; thrombocytopenia
Caused by shiga-like toxin (STX) from E. coli
NO antibiotics (increase toxin production) Treat with fluid management to protect kidney
Briefly list the steps of EHEC pathogenicity.
EHEC cells remain extracellular in gut (no bacteremia) - TOXIN gets into bloodstream
- Attachment via common pillus -> effacement
- T3SS mediated change in host cells by injection of Tir -> Tir-Intimin binding -> tight interaction
- Actin polymerization and pedestal formation
- Release of HEMOLYSIN, SHIGA-like toxin, and other factors
Why can animals carry EHEC without getting sick?
Shiga toxin binds a host glycolypid that is different between cattle and humans
What does EPEC cause? How is it transmitted?
Childhood diarrhea (MANY cases) Transmitted person to person
What does ETEC cause? How is it transmitted?
Traveler’s diarrhea, esp. in infants in developing word
Food/water
Person to person
What E. coli type causes the most epidemics?
STECs
Describe how EHEC binds to the gut, including virulence determinant involved.
Uses E. coli common pilus (same as commensal E. coli)
What is the pathogenicity island in EHEC? What does it encode
LEE = Locus of Enterocyte Effacement
- -Encodes T3SS and effectors
- -Encodes Tir and Intimin
How does the T3SS in EHEC function?
Induced by many things, including contact with host cell
EspA = needle
EspB/D pass through needle to tip -> form pores in eukaryotic membrane for proteins to pass through
How is the attachment of the T3SS in EHEC to the host cell promoted? Why? (know this)
Promotes attachment by creating its own receptor:
- -Injects TIR into host cell -> exposed on host cell surface
- -Expresses INTIMIN on its own membrane
- -Tir and Intimin interact with each other, binding tightly
Doesn’t have much time, needs to attach to wall as it is moving along - this helps it attach faster