DSBs

Question 1

DSBs are the…………..

Cells cant….

Answer 1

Most dangerous type of DNA damage

Survive even a single DSB in the absence of repair

Question 2

If not repaired or repaired incorrectly:

Answer 2

Mutations and/or chromosomal aberrations are induced

These can lead to cell death or cancer

Question 3

Topoisomerases are:

Answer 3

Transesterases

They make DSB and become covalently attached to the ends

Question 4

What family does DNA-PKcs belong to?

Answer 4

PIKK

Family also includes ATM and ATR

Question 5

The kinase domain of DNA-PK is in the

Answer 5

C terminal domain

Question 6

Adding phosphate to a protein adds……. which can increase or decrease ability to……….

Answer 6

Charge

Interact with partner proteins

Question 7

VDJ recombination

Answer 7

Variable diversity and joining recombination

Early B and T cells

Generate antigen receptor genes

Question 8

CSR

Answer 8

Class switch recombination

Occurs only in B cells after they have completed VDJR to change from IgN to IgG/E/A

Question 9

Which is more accurate: HR or NHEJ?

Answer 9

HR-uses undamaged DNA template to repair via base pairing

Question 10

Common step in all HR pathways

Answer 10

SsDNA formation at DSB end

Question 11

How is the 3’ extension created and where?

Answer 11

Either side of the break

By removing 5’ ending strands