Draize Test Flashcards
Serve as standard for evaluation of local toxicity. It may also serve as an acute toxicity test for cosmetic products
Draize Skin Irritation Test
Test animal model
Rabbits
Why rabbits?
- Sensitive skin compared to human skin
- Rabbit skin has high permeability
- Easier to handle
- Well-described anatomy: big eyes and large surface area
*Low cost and readily available
Protocols 24 hours before the test
- Hair removal by clipping or shaving
- Avoid abrading the skin
- 10% of the area should be clear
T/F: The weight of the animal should be the deciding factor on the area and dimensions to be cleared
True
A test where an allergen is applied to skin under occlusion and the skin is observed for delayed reaction (generally type IV)
Patch Test
First evaluation: after 24 hours; Second evaluation: After ___ hours
72
A test to determine irritation and inflammation
Scratch test
In the scratch test, the first step is to abrade the skin by slightly scratching it ___ to ___ times using 20G hypodermic needle
5 to 7
Redness of skin as a results of increased local blood flow
Erythema
Accumulation of fluid in subcutaneous and interstitial spaces
Edema
Is a dead tissue that sheds or falls off from healthy skin. It is a dry necrotic tissue that sloughs off
Eschar
T/F: Test sample is not a primary skin irritant if primary irritation index is less than 6.
False. Test sample is not a primary skin irritant if primary irritation index is less than 5
Draize Skin Irritation Test Interpretation
0 - 0.03
No irritation
Draize Skin Irritation Test Interpretation
0.04 - 0.99
Irritation barely perceptible
Draize Skin Irritation Test Interpretation
1.00 - 1.99
Slight irritation
Draize Skin Irritation Test Interpretation
2.00 - 2.99
Mild irritation
Draize Skin Irritation Test Interpretation
3.00 - 5.99
Moderate irritation
Draize Skin Irritation Test Interpretation
6.00 - 8.00
Severe irritation
This part of the eye controls the amount of light entering the eye by opening and closing the pupils
Iris
This part of the eye has no blood vessels, and it can be transplanted
Cornea
This part of the eye lubricates the eye
Conjunctiva
Protocols for the Draize Eye Irritation Test
T/F: Topical ocular anesthetic should be applied at least 30 minutes before
False. Apply topical ocular anesthetic (5 mins before)
T/F: Place/Apply test substance in the conjunctival sac of one eye
True
T/F: The eyes of test animals should not be washed for at least 24 hours
True
Observe and score the eyes of the test animals after ___, ___ and ___
hours.
24, 48, 72
Conjunctival edema/ swelling
chemosis
Draize Eye Irritation Test Interpretation
0 - 0.9
Non-irritating
Draize Eye Irritation Test Irritation
1 - 25
Mild
Draize Eye Irritation Test Interpretation
26 - 56
Moderate
Draize Eye Irritation Test Interpretation
57 - 84
Severe
Draize Eye Irritation Test Interpretation
85 - 110
Very severe
