Draize Test Flashcards

1
Q

Serve as standard for evaluation of local toxicity. It may also serve as an acute toxicity test for cosmetic products

A

Draize Skin Irritation Test

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2
Q

Test animal model

A

Rabbits

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3
Q

Why rabbits?

A
  • Sensitive skin compared to human skin
  • Rabbit skin has high permeability
  • Easier to handle
  • Well-described anatomy: big eyes and large surface area
    *Low cost and readily available
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4
Q

Protocols 24 hours before the test

A
  • Hair removal by clipping or shaving
  • Avoid abrading the skin
  • 10% of the area should be clear
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5
Q

T/F: The weight of the animal should be the deciding factor on the area and dimensions to be cleared

A

True

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6
Q

A test where an allergen is applied to skin under occlusion and the skin is observed for delayed reaction (generally type IV)

A

Patch Test

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7
Q

First evaluation: after 24 hours; Second evaluation: After ___ hours

A

72

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8
Q

A test to determine irritation and inflammation

A

Scratch test

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9
Q

In the scratch test, the first step is to abrade the skin by slightly scratching it ___ to ___ times using 20G hypodermic needle

A

5 to 7

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10
Q

Redness of skin as a results of increased local blood flow

A

Erythema

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11
Q

Accumulation of fluid in subcutaneous and interstitial spaces

A

Edema

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12
Q

Is a dead tissue that sheds or falls off from healthy skin. It is a dry necrotic tissue that sloughs off

A

Eschar

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13
Q

T/F: Test sample is not a primary skin irritant if primary irritation index is less than 6.

A

False. Test sample is not a primary skin irritant if primary irritation index is less than 5

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14
Q

Draize Skin Irritation Test Interpretation

0 - 0.03

A

No irritation

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15
Q

Draize Skin Irritation Test Interpretation

0.04 - 0.99

A

Irritation barely perceptible

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16
Q

Draize Skin Irritation Test Interpretation

1.00 - 1.99

A

Slight irritation

17
Q

Draize Skin Irritation Test Interpretation

2.00 - 2.99

A

Mild irritation

18
Q

Draize Skin Irritation Test Interpretation

3.00 - 5.99

A

Moderate irritation

19
Q

Draize Skin Irritation Test Interpretation

6.00 - 8.00

A

Severe irritation

20
Q

This part of the eye controls the amount of light entering the eye by opening and closing the pupils

A

Iris

21
Q

This part of the eye has no blood vessels, and it can be transplanted

A

Cornea

22
Q

This part of the eye lubricates the eye

A

Conjunctiva

23
Q

Protocols for the Draize Eye Irritation Test

T/F: Topical ocular anesthetic should be applied at least 30 minutes before

A

False. Apply topical ocular anesthetic (5 mins before)

24
Q

T/F: Place/Apply test substance in the conjunctival sac of one eye

A

True

25
Q

T/F: The eyes of test animals should not be washed for at least 24 hours

A

True

26
Q

Observe and score the eyes of the test animals after ___, ___ and ___
hours.

A

24, 48, 72

27
Q

Conjunctival edema/ swelling

A

chemosis

28
Q

Draize Eye Irritation Test Interpretation

0 - 0.9

A

Non-irritating

29
Q

Draize Eye Irritation Test Irritation

1 - 25

A

Mild

30
Q

Draize Eye Irritation Test Interpretation

26 - 56

A

Moderate

31
Q

Draize Eye Irritation Test Interpretation

57 - 84

A

Severe

32
Q

Draize Eye Irritation Test Interpretation

85 - 110

A

Very severe