Dr Wetherly Flashcards
What is homogenisation
Where the cells are broken up using a blender releasing organelles from a cell
What is a Detergent
Something that would damage proteins
What is a buffer
Stops pH from fluctuating
What is Ultracentrifugation
Where the fragments in the homogeniser are separated in a machine called a centrifuge
Why is the material kept cool during cell fractionation
To reduce enzyme activity that might break down the organelles
Why must the solution be isotonic in cell fractionation
To prevent osmotic in the cell
Osmotic =
Organelles bursting or shrinking
Why is the solution filtered in cell fractionation
To remove any debris
What are the 6 stages in the cell cycle
Interphase Prophase Metaphase Anaphase Telophase Cytokinesis
What happens in Interphase in the cell cycle:
Cell is replicating DNA
Can’t see chromosomes yet
What happens in Prophase in the cell cycle:
Chromosomes visible
Nuclear envelope and nucleolus disintegrated
What happens in Metaphase in the cell cycle:
Chromosomes line up in centre of cell
Spindles form
Spindles attract to chromosomes
What happens in Anaphase in the cell cycle:
Spindle fibres contrast
Chromosomes pulled to opposite side
What happens in Telophase in the cell cycle:
Nuclear envelope reforms around two sets of chromosomes
Nucleolus reforms
Spindles disintegrates
What happens in Cytokinesis in the cell cycle:
Cytoplasm divides
Cell divides
Two genetically identical cells
