Donor Screening/Component Prep

Question 1

What are the facilities a blood bank lab is involved in?

Answer 1

Collection
Storage
Processing
Blood distribution

Question 2

What MUST a blood bank do upon receiving a shipment of blood?

Answer 2

Reconfirm ABO label
Retype all negative Rh’s

Question 3

How many identifiers are required for patient samples?

Answer 3

Two
Full name/DOB

Question 4

Once we deem a patient sample acceptable, what are the types of tests we perform?

Answer 4

Type & Screen
Type & Crossmatch
Prenatal Eval
Postpartum Eval
Cord Blood

Question 5

Who provides quality blood products for service members, veterans, and families?

Answer 5

Armed Services Blood Program (ASBP)

Question 6

What does a standard BLDREP contain?

Answer 6

Current Blood inventory
Blood products required with 12-48 hrs

Question 7

What is included in a Blood Shipment Report (BLDSHIPREP)?

Answer 7

Receiver of blood products
Schedule of shipped blood
Type of shipment
Address and POC

Question 8

Define an Allogenic Donor.

Answer 8

One who donates blood for others to use

Question 9

Who governs allogenic and autologous donations?

Answer 9

AABB
FDA

Question 10

What is the min. and max. age limit for donating allogenic blood?

Answer 10

Min: 17 years old
Max: None

Question 11

What is the time interval for an allogenic blood donor?

Answer 11

Eights weeks or 56 days
48 hours if done through pheresis

Question 12

What kind of deferral would we determine if a patient has recently had surgery or had components transfused during surgery?

Answer 12

Temporary Deferral
12 months

Question 13

What kind of deferral would we determine if a donor has taken piroxicam, aspirin, or anything containing aspirin?

Answer 13

Temporary Deferral
Inhibited PLT function
3 day deferral

Question 14

What is a permanent deferral?

Answer 14

A donor that will never be eligible to donate for someone else
Eligible for autologous donation

Question 15

What involves collecting blood from the surgical site or reinfusing lost blood during surgery?

Answer 15

Intraoperative collection

Question 16

What is Apheresis?

Answer 16

Automated cell separator using centrifugation
Based on differences in density

Question 17

What is Apheresis used to collect?

Answer 17

PLT’s
Plasma
WBC’s
RBC’s
Stem cells

Question 18

What Pheresis method defers donors taking Feldene, aspirin, Plavix, or Ticlid?

Answer 18

Plateletpheresis
Feldene/Aspirin: 48 hour deferral
Plavix/Ticlid: 14 days

Question 19

Which Pheresis method involves the removal and retention of plasma such as antibody, toxin, abnormal proteins?

Answer 19

Plasmapheresis

Question 20

Which Pheresis method defers donors for 16 weeks following a successful completion?

Answer 20

Double RBC Pheresis

Question 21

How does Membrane Technology Methodology function?

Answer 21

Uses centrifugal force to separate blood based on density

Question 22

What are the most common problems encountered in Apheresis procedures?:

Answer 22

Citrate Toxicity
Vascular Access Complications
Vasovagal Reactions
Hypovolemia

Question 23

What technique involves scrubbing a 4 cm area in all directions for a min of 30 seconds?

Answer 23

Aseptic Technique

Question 24

What are signs of a mild reaction to transfusions?

Answer 24

Convulsions
Sweating
Dizziness
Pallor

Question 25

What are signs of a moderate reaction to transfusions?

Answer 25

Loss of Consciousness Systolic pressure fall (60 m Hg)

Question 26

How can we treat a moderate reaction to transfusions?

Answer 26

Administer 95% O and 5% CO2

Question 27

What are signs of a severe reaction to transfusions?

Answer 27

Convulsions: Cerebral Ischemia Hyperventilation Epilepsy

Question 28

How can we treat a severe reaction to transfusions?

Answer 28

Call for help Notify physician Restrain donor

Question 29

What state is blood stored in?

Answer 29

Liquid state (1C - 6C)

Question 30

What is CPDA-1 (Citrate-Phosphate-Dextrose-Adenine) and how long can we store it?

Answer 30

An Anticoagulant preservative 35 days of storage

Question 31

What can additive solutions do to pRBC's?

Answer 31

Extend the shelf life to 42 days More efficent harvest of plasma and PLT's

Question 32

What are the different types of additive solutions we can use?

Answer 32

Adsol (AS-1) Nutricel (AS-3) Optisol (AS-5)

Question 33

What additive contains citrate and phosphate?

Answer 33

Nutricel (AS-3)

Question 34

What method is used to measure Hepatitis B Surface Antigen (HBsAg)?

Answer 34

Enzyme-Linked Immunosorbent Assay (ELISA)

Question 35

What method is used to screen Hepatitis C?

Answer 35

Enzyme Immunoassay (EIA)

Question 36

What test do we use to confirm Hepatitis C?

Answer 36

RadioImmunoBlot Assay (RIBA)

Question 37

What must we screen all donor units for using the EIA method?

Answer 37

Presence of HIV 1 or 2 antibody

Question 38

What can we use to confirm the HIV 1/2 screen?

Answer 38

Western Blot (Wb) Immunoflouresence (IFA)

Question 39

What is a donation number that consists of 14 characters?

Answer 39

ISBT number

Question 40

What is the QC regarding WBC in Leukoreduced RBC's?

Answer 40

WBC <5 x 10^6

Question 41

What are some indications of Leukoreduced RBC's?

Answer 41

Febrile Nonhemolytic Transfusion Reaction Transmission of Cytomegalovirus

Question 42

What temp do we store PLT's at?

Answer 42

20-24C with continuous agitation

Question 43

What is the shelf life of Cryoprecipitate?

Answer 43

Remove from plasma Store at -18C Expires 1 year after collection

Question 44

What is the shelf life of RhoGam?

Answer 44

Store at 1-6C with expiration date IM preparations: 50ug, 300ug

Question 45

Under what conditions would we freeze/rejuvenate RBC's?

Answer 45

Patients with rare phenotypes Autologous use Frozen with glycerol 10 year storage

Question 46

Which freezing method is at -80C, freezes RBCs within 6 days, and is the most widely used procedure?

Answer 46

High glycerol method

Question 47

Which freezing method is at -120C and is 20% weight per volume?

Answer 47

Low glycerol method

Question 48

Which process restores ATP and 2,3 DPG?

Answer 48

Rejuvenation of RBCs

Question 49

What is the purpose of the Military Frozen Blood Program?

Answer 49

Long term storage Increase RBC utilization Decrease post transfusion hepatitis

Question 50

What are the shipping procedures for non frozen blood?

Answer 50

Up to 30 liquid RBC units Cover products with 14 lbs of cubed wet ice