Dodd Extra reading

Question 1

Fenske and Imaizumi, 2016

Answer 1

• Plants use scent at specific times of day to coincide with pollinator foraging
Petunia hybrida - FVBP major metabolic pathway.
TFs ODO1, EOB1, EOB2 have diurnal nRNA expression, pos regulators for genes in pathway.
PhLHY homologue of arabi oscillator LHY gene.
PhLHY negative regulator of scent, upreg in morning.
found expression of different scents (eg methyl benzoate) at different times - antiphasic, suggesting different mechanisms for each compound emission.
flowers can generate own C rhythm.

Question 2

how does scent pathway go in P hybrida?

Answer 2

Femske and Imaizumi 2016
expression of ODO1 - first regulatory component peaks in evening, before night scent release. constitutiv expression of PhLHY= complete loss of scent emission, PhLHY binds to ODO1.
RNAi suppression of ODO1 - reduction in all scent emission.
ODO1 suppression also reduces transcript of many FVBP genes, many are upstream suggesting FVBP controls more than just scent emission.

EOB2 is second regulatory component. suppression decreased emission of most volatiles.

Question 3

What was in Wang et al, 2016, C4 rice project

Answer 3

Could meet predicted demand for 50% increase in yield by 2050
3types ofleaf anatomy for c4:
- single cell - distinct chloroplasts partitioned in distinct cytoplasmic compartments within cell.
- Multiple cells - kranz anatomy
- inducible - in certain conditions metabolism and chloroplast partitioned i
nto dimorphic cells.

compared transcriptomes of sp in Cleome and Flaveria, and also btw tissues of same plant, and at diff dev stages.
Used expected C4 pathway enzyme expression profiles toset bioinformatic filters tofind C4 associated genes.

found upreg in C4: transporter proteins to move int btw M and BS cells. genes dedicated toATP porduction,

Comparisons of evolutionary trajectories of C3 to C4 transition. must have been many small scale changes, not just one master regulator change.

Question 4

Richards et al 2012

Answer 4

time series transcriptomic data, of A thaliana in GH
obtained 15352 genes at 9 timepoints.
growth stage - rep/veg affected transcriptome the most.

Question 5

Kudoh 2016 - High resolution molecular phenology data

Answer 5

FLC silencing maintained until end of annual life in A. thaliana, but if not cold for long enough, FLC can be reactivated, showing memory not wiped.
used HMP data to determine length of memory in perennials which can reverse suppression of FLC after long periods of cold.