doc father rem Flashcards
other term for fecal exam
- fecalysis
- coproscopic exam
scoring system
- Waltham (1-5; hard to soft)
- Veal (0-3; hard to soft)
factors that may affect fecal exam
- amount
- age of sample
- sample handling (collection, storage, transpo)
- examination method
- skill
required g of fecal sample
5-10 g (thumb-size)
if fecal sample needs to be refrigerated, what temp?
3-5C
- do not freeze because it can distort parasite eggs
- should be examined w/in 7 days of collection
T/F: fecal sample can be preserved using 5-10% formalin
T
qualitative fecal exam
- dfs
- flotation
- sedimentation
- larval recovery
quantitative fecal exam
- stoll-ova counting technique
- McMaster egg-counting technique
- Beaver’s direct egg-counting technique
SG of nematode and cestode egg
1.10-1.20
SG of trematode egg
1.30-1.35
common flotation sol’ns
- NaCl
- common helminths
- protozoan ova and cysts - Sheather’s
- common helminths
- protozoan (esp. Cryptosporidium oocysts) - Sodium nitrate
- common helminths
- protozoan ova and cysts - zinc sulfate
- common helminths (esp. Giardia)
- protozoan (esp. lungworm larvae) - magnesium sulfate
- common helminths
- protozoan
T/F: if flukes are suspected, sedimentation is advised
T
T/F: NaCl, sodium nitrate, and magnesium sulfate distort Giardia rapidly
T
type of flotation where a small amount of feces is mixed thoroughly w/ flotation solution, filling the tube w/ flotation sol’n up to the brim then a cover glass is placed on top of tube or vial and suspension is allowed to stand for 10-15 min
passive flotation
type of flotation that facilitates faster flotation and recovers more eggs and oocysts
centrifugation flotation
method of choice if trematode infxn is suspected, including Fasciola, Dicrocoelium, Paramphistomum, Schistosoma
sedimentation
T/F: sedimentation’s concentration ability is higher than flotation
F - lower
useful for isolation and identification of ovoviviparus nematodes and strongyles
Baermann technique
other fecalysis techniques
- coproantigen tests - detects Ag
- Ab detection test for Ostertagia and F. hepatica
- plasma pepsinogen test - diagnosis of abomasal or stomach damage due to trichostrongyles
- PCR
T/F: thick smears are more concentrated and offer higher sensitivity than thin smear, but morphological ID may be difficult
T
T/F: capillary blood sample (ear tip) is more useful when looking for Babesia
T
stains that are more preferred for examining protozoan and rickettsiae
- Romanowsky (Wright and Giemsa)
T/F: D. immitis is motile but stays in same place
T
T/F: Acanthocheilonema is highly motile, swimming thru the blood
T
