DNA Technology Flashcards
How Recombinant DNA technique is done?
This is acheived by using specific restriction enzyme for cutting the DNA INTO SUITble fragments and then joining together the appropriate fragments using DNA ligase enzyme.
Purpose of Recombinant DNA technique?
1- to obtain large no. of copies of specific DNA fragments
2- to recover large quantities of the protein produced by the concerned gene
3- to integrate the gene into the chromosome of the target org.
What is a Restriction enzyme?
Is an enzyme that cuts the dsDNA at specific recognition nucleotide sequences known as restriction sites.
Restriction enzyme are found in_____?
Bacteria
Applications of Restriction enzyme?
- Used to assist insertion of genes into plasmid vectors during gene cloning and protein expression experiments.
- used to digest genomic DNA for gene analysis by southern blot
- used to distinguish gene alleles
What is a ‘vector’?
Is an agent that can carry a DNA fragment into host cell.
What is ‘ cloning vector’?
If the vector is used for reproducing the DNA fragment,it is called a ‘cloning vector’
What is a ‘expression vector’?
If vector is used for expressing certain gene in the DNA fragment
The DNA segment,to be cloned is called_____?
DNA insert
Write steps of Recombinant DNA TECH/ gene cloning?
1- identification and isolation of the desired gene or DNA fragment to be cloned.
2- insertion of isolated gene in a suitable vector
3- introduction of this vector into a suitable org./ cell called host ( transformation )
4- selection of the transformed host cells
5- multiplication/ expression/ integration followed byexpression of the introduced gene in the host.
Explain Recombinant DNA technique?
Recombinant DNA technique otherwise called as gene cloning is the technique which facilitate the formation of chimeric DNA by fusing DNAs from different sources.
