DNA tech vectors

Question 1

Describe the insert size, number of clones, way of replication, host organism, way of entering the host and application for Plasmids

Answer 1

• insert size is 5kb
• # of clones is 1.7 x 10^6 (E.coli vector)
• replicate independently of chromosomes
• host organism is e.coli
• Enter host via transformation
• Used as cloning vectors to clone your favourite sequence, expression of CDS.

Question 2

Describe the insert size, number of clones, way of replication, host organism, way of entering the host and application of phagemids

Answer 2

• insert size is 10kb
• # of clones is
• Replicate from f1 phage and contain f1 origin
• Host organism is E.coli
• Enter host via transformation
• Application is type of cloning vector with M13 and discovered therapeutic monoclonal Abs, allows finding protein of desired characterstics from library

Question 3

Describe the insert size, number of clones, way of replication, host organism, way of entering the host and application of Lambda phage

Answer 3

• Insert size - 20kb
• # of clones is 4.5 x 10^5
• Replicates firstly by theta, then by rolling circle forming concatemers (both strands at same time)
• E.coli
• Enters cell by transfection
• Application used to clone recombinant DNA by transfection, linear dsDNA.

Question 4

Describe the insert size, number of clones, way of replication, host organism, way of entering the host and application of Cosmid

Answer 4

• insert size 50kb
• # of clones is 1.8x10^5
• Replicates by theta or rolling circle, depending on vector
• host E.coli
• enters host by transformation of empty vector, packaging and infection for recombinants
• Application eukaryotic genomic libraries or metagenomic libraries, derived from lambda

Question 5

Describe the insert size, number of clones, way of replication, host organism, way of entering the host and application of BAC

Answer 5

• insert size 150kb
• # of clones 6 x 10^4
• replication by theta or rolling circle depending on plasmid
• E.coli
• Application is eukaryotic genomic libraries as it is bacterial artificial chromosome, they are unstable and jhave phased out though

Question 6

Describe the insert size, number of clones, way of replication, organism host way of entering the host and application of YAC

Answer 6

• insert size 1000kb
• # of clones 9 x 10^3
• Organism host bakers yeast
• Introduction of host is transformation
• Application eukaryotic genomic libraries, unstable and have phased out.