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Biochemistry 2 > DNA Tech > Flashcards

DNA Tech Flashcards

1
Q

Restriction Endonuclease

A

cuts double stranded DNA at specific sites

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2
Q

DNA fingerprinting

A

the number of minisatellite or microsatellite repeats vary from one individual to another

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3
Q

Restriction site

A

specific sequence of bases cleaved by a restriction enzyme. Commonly 4-6bp and palindromic

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4
Q

Vector

A

vehicles that carry DNA fragments into cells

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5
Q

Blunt end

A

cutting by restriction endonuclease yields dsDNA with no overhang

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6
Q

Transduction

A

phage mediated gene transfer into prokaryotic cell

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7
Q

Sticky end

A

cutting by restriction endonuclease yields dsDNA WITH an over hang on one of the strands

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8
Q

Transformation

A

non-phage mediated gene tranfer into prokaryotic cell

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9
Q

DNA ligase

A

join together(ligate) frag of DNA. Repair single strand breaks in vivo, repair ds breaks in vitro

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10
Q

Transfection

A

gene transfer into eukaryotic cell

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11
Q

Linker

A

dsDNA added to blunt ends to make them sticky

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12
Q

Recombinant DNA molecule

A

a vector with incorporated gene

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13
Q

DNA polymerase

A

makes copies of DNA by synthesizing a single complementary strand to an existing single-stranded template

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14
Q

Clone

A

groupe of organisms, cells or moeluces all from single individuals

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15
Q

Reverse Transcriptase

A

synthesizes a DNA strand complementary to an RNA template - Makes cDNA

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16
Q

Genomic library

A

collection of DNA frags from an organisms genome

17
Q

Hybridization

A

annealing of two single strands

18
Q

cDNA library

A

collection of cDNA fragments - produced form all present mRNA

19
Q

Probe

A

short single stranded nucleotide of knmown sequence that is labeled to allow idenfitication

20
Q

PCR

A

polymerase chain reaction - in vitro method for producing large amounts of DNA from target seq

Biochemistry 2 (3 decks)

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