DNA Sequencing

Question 1

When was Sanger sequencing invented?

Answer 1

1977

Question 2

What method does Sanger sequencing use?

Answer 2

Chain termination method - Dye terminating sequencing

Question 3

What is the read length of Sanger sequencing?

Answer 3

700-900bp

Question 4

What is the fidelity of Sanger sequencing?

Answer 4

High

Question 5

What were some benefits of Next Generation Sequencing?

Answer 5

• Cheaper, Faster, more Accurate
• 1 Human genome per day capacity
• Higher depth of sequencing

Question 6

How much coverage do we need to determine the genome of an organism that we do not already know?

Answer 6

80x - This is called de novo sequencing

Question 7

How much coverage do we need to determine the genome of an organism, when we have a reference genome?

Answer 7

10-30x - This is called resequencing

Question 8

What does the Oxford Nanopore do?

Answer 8

Single molecule sequencing

Question 9

What are some sources of noise in DNA sequencing?

Answer 9

DNA sampling bias
Library prep bias
PCR Primer bases
Homopolymer
GC content coverage bias