DNA sequencing Flashcards
In the procedure indicated by letter A, DNA segments from humans and bacteria are joined by the action of?
Enzymes
The accompanying diagrams represent some steps in a procedure used in biotechnology.
Biochemical catalyst involved in the insertion of genes into other organisms
The accompanying diagram illustrates some key steps of a procedure in one area of biotech
Bacterial cells that are able to synthesize insulin
The result of this type of DNA analysis is often used to help determine
if two species are closely related
During replication, the strands of a double-stranded DNA molecule, separate from each other when bonds are broken.
Nitrogenous bases
Which nucleotide contains a nitrogenous base that pairs with guanine in double-stranded DNA
4
Identify the enzyme that is used to separate DNA strands during DNA replication.
Helicase
Okazaki fragments are formed on which strand of DNA
Lagging Strand
Which step in PCR is responsible for adding primers to the DNA strand?
Annealing
which step in PCR is completed at the highest temperature in the thermocycler?
Denaturation
what are the appropriate steps when extracting DNA?
- Smash Cell
- Sort DNA from other fragments
- Precipitation
4.Enzyme target specific site
Gel electrophoresis is used to separate DNA fragments on the basis of their ….
size
In what part of DNA is genetic information located
Nucleotide sequence
what factor causes the DNA fragments to move this technique
size speed and heat
