DNA RNA and protein systensis Flashcards
Describe the structure of DNA
a chain of nucleotides composed of dexoribose sugar, phosphate and an organic base (adenine, guanine, cytosine and thymine)
What are purines
Adenine and Guanine - double ring structures
What are pyrimidines
Thymine and Cytosine - single ring structures
Complementary base pairings
bases that will pair together to hold DNA strands together by hydrogen bonds
*cytosine - guanine
*Adenine - thymine
How does the structure of DNA relate to its function
*complementary base pairings - allows the DNA to replicate itself and copy the same information to mRNA
*weak hydrogen bonds - allow the strands to separate
*it’s compact and long - large amounts of coded information and can fit inside the nucleus of the cell
Describe the structure of RNA
a single stranded chain of nucleotides made of a ribose sugar, phosphate and a base (Adenine, Uracil, Guanine, Cytosine) held together by phosphodiester bonds
what are the 3 types of RNA
*mRNA - moves the genetic information from the inside of the nucleus to the cytoplasm
*tRNA - carries the correct amino acid into the sequence of a polynucletide
*rRNA - part of the ribosome
structure of mRNA
short single stranded chain of RNA polynucleotide
Structure and function of tRNA
structure - single stranded, cloverleaf shape, contains 2 regions : anticodons, amino acid attachmenr site
Function - the anticodons allow the tRNA to bring the correct amino acids
what are genes
a sequance of DNA that codes for the production of specifc proteins
What is ment by the DNA code being degenerate
There is more than one code for each amino acid. There are 20 amino acids and 64 possible combinations
What is ment by the DNA code being non-overlapping
Each base is present in only 1 codon
What is ment by the DNA code being universal
Each codon is the code for the same amino acid in all living organism
Where does transcription occur
in the nucleus
what does transcription produce
pre - mRNA
Describe the process of transcription
1) the hydrogen bonds between the 2 strands are broken causing the 2 strands to seperate and the nucleotide bases to be exposed
2) RNA polymers bind to the template strand at the start of the sequance, the template strand is complemetary to the coding strand
3) complementary RNA nucleotides join to the template strand and RNA polymerase moves along the template joining RNA nucleotides together with phosophodiester bonds 4
4) when the RNA polymerase reaches a STOP codon the pre-mRNA strand is released and the DNA recoils
Describe the process of splicing
The removal of introns from pre-mRNA and binding of the exon ends by a spllicosome in order to make mRNA
Where does translation occur
at the ribosome in the cytoplasm
How many codons can a ribosome hold
2
What is the start codon
AUG
Describe the process of translation
1) the mRNA binds to the ribosome
2) the tRNA with the complemenatry anticodon to the mRNA codon brings a specific amino acid to the ribosome
3) a second tRNA molecule brings a second amino acid to the ribosome
4) a peptide bond is formed between the 2 amino acids
5) the ribosomes move along 1 codon and the first tRNA molecule is released back into the cytoplasm to bring another amino acid
6) the process contines untill a stop codon is reached and the polypeptide chain is released.