DNA replication prokaryotes review Flashcards
1
Q
oriC
A
site where DNA replication begins
2
Q
DnaA
A
opens DNA to allow dnaB to bind
3
Q
DnaB
A
helicase, uses ATP
4
Q
DnaC
A
loads DnaB (helicase)
5
Q
DNA primase
A
RNA polymerase that adds DNA primer
6
Q
DNA pol III
A
main pol in prokaryotes, adds 5-3, proofreads 3-5
core: alpha, epison, theta
7
Q
sliding clamp
A
positions pol III on DNA, beta
8
Q
clamp loader
A
loads sliding clamp, gamma
9
Q
DNA gyrase
A
=topoisomerase II
negative supercoils
uses ATP
10
Q
RNase H
A
removes RNA primer
11
Q
DNA pol I
A
5-3 exonuclease activity, replaces RNA primer spot with DNA
12
Q
DNA ligase
A
seals nick between nucleotides (ATP)
13
Q
Tus
A
binds termination sequence, prohibits helices from advancing
14
Q
core enzyme subunits in pol III
A
alpha, epison, theta
15
Q
tau subunit of pol III
A
dimerizes pol III complexes of leading and lagging strands
