DNA Replication Flashcards

1
Q

E. Coli - Origin of Replication

A

OriC

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2
Q

Yeast - Origin of Replication

A

ARS - Autonomous Replicating sequences

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3
Q

E. Coli - Binds Origin of Replication

A

dnaA complex

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4
Q

Yeast - Binds Origin of Replication

A

ORC - Origin of Replication Complex

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5
Q

E. Coli - Stabilize single stranded DNA

A

SSB’s

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6
Q

Yeast - Stabilize single stranded DNA

A

RPA - Replication Protein A

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7
Q

E. Coli - Increase Processivity

A

Beta subunit of Polymerase III

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8
Q

Yeast - Increase Processivity

A

PCNA - Proliferating cell nuclear antigen for DNA polymerase Delta

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9
Q

E. Coli - Clamp loader

A

gamma complex

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10
Q

Yeast - Clamp loader

A

RFC - replication factor C

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11
Q

E. Coli - Make RNA Primer

A

dnaG

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12
Q

Yeast - Make RNA Primer

A

DNA polymerase alpha

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13
Q

E. Coli - Synthesize DNA

A

DNA polymerase III

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14
Q

Yeast - Synthesize DNA

A

DNA polymerase epsilon (leading strand)
DNA polymerase delta (lagging strand)
DNA polymerase gamma (mitochondria)

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15
Q

E. Coli - Removal of RNA Primers

A

DNA Polymerase I

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16
Q

Yeast - Removal of RNA Primers

A

Rnase H and Fen1 (flap endonuclease 1)

17
Q

E. Coli - Helicase

A

dnaB/dnaC complex

18
Q

Yeast - Helicase

A

MCM - mini chromosome maintenance proteins