DNA replication Flashcards

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1
Q

when does DNA replication occur in the cell cycle ?

A

it occurs before mitosis, in the S- phase

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2
Q

why does DNA replication occur before mitosis ?

A

because before the cell divides all the DNA must replicate to provide a copy for the new cell

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3
Q

the process of DNA replication is described as… ?

A

semi-conservative method

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4
Q

why is DNA replication described as semi-conservative ?

A

because in the daughter DNA , one strand is from the original, parental DNA and one strand is newly synthesised

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5
Q

which enzymes are involved in DNA replication ?

A

DNA helicase
DNA polymerase

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6
Q

step 1 of DNA replication

A

DNA helicase breaks the hydrogen bonds between the complementary base pairs between the 2 strands whithin a double helix
this causes the DNA double helix to unwind

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7
Q

what is meant by parental DNA ?

A

name given to the original DNA that is gong to be copied

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8
Q

step 2 of DNA replication

A

each of the separated parental DNA strands acts as a template
free floating DNA nucleotides within the nucleus are attracted to their complementary base pairs on the template strands of the parental DNA

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9
Q

step 3 of DNA replication

A

the adjacent nucleotides are joined to form the phosphodiester bond by a condensation reaction
- DNA polymerase catalyses the joining together of adjacent nucleotides

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10
Q

step 4 of DNA replication

A

the 2 sets of daughter DNA contains one strand from the parental DNA and one newly synthesised strand

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