DNA replication Flashcards
when does DNA replication occur in the cell cycle ?
it occurs before mitosis, in the S- phase
why does DNA replication occur before mitosis ?
because before the cell divides all the DNA must replicate to provide a copy for the new cell
the process of DNA replication is described as… ?
semi-conservative method
why is DNA replication described as semi-conservative ?
because in the daughter DNA , one strand is from the original, parental DNA and one strand is newly synthesised
which enzymes are involved in DNA replication ?
DNA helicase
DNA polymerase
step 1 of DNA replication
DNA helicase breaks the hydrogen bonds between the complementary base pairs between the 2 strands whithin a double helix
this causes the DNA double helix to unwind
what is meant by parental DNA ?
name given to the original DNA that is gong to be copied
step 2 of DNA replication
each of the separated parental DNA strands acts as a template
free floating DNA nucleotides within the nucleus are attracted to their complementary base pairs on the template strands of the parental DNA
step 3 of DNA replication
the adjacent nucleotides are joined to form the phosphodiester bond by a condensation reaction
- DNA polymerase catalyses the joining together of adjacent nucleotides
step 4 of DNA replication
the 2 sets of daughter DNA contains one strand from the parental DNA and one newly synthesised strand