DNA Replication Flashcards
1
Q
describe the importance of DNA replication
A
- life begins as one cell and moves onward
- errors causes problems in cell division, which can lead to disease and possible death
2
Q
what is semiconservative replication
A
each daughter strand has half parent and half new DNA
3
Q
in what direction is new DNA made
A
5’ to 3’ direction
4
Q
how are both new DNA strands made in the 5’ to 3’ directions
A
through leading and lagging mechanisms
5
Q
what is the significance of DNA polymerase
A
- synthesizes new DNA
- monitors for correct base-pairing between incoming nucleotides
- proofreading/self-correcting
6
Q
describe the general process of DNA synthesis
A
- DNA polymerase needs base-paired nucleotides
- DNA primase makes small region of RNA for DNA polymerase to begin
- nuclease breaks RNA primer
- Repair polymerase replaces RNA with DNA
- DNA ligase joins in small grooves in DNA backbone
7
Q
what are SSBPs
A
- single-stranded binding proteins
- binds unwound single stranded DNA
- prevents base-pair formation
8
Q
what is the purpose of the sliding clamp
A
to keep DNA polymerase attached to template
