DNA Replication

Question 1

What is the DNA replication ?

Answer 1

The synthesis of DNA molecule during cell division and transmission (transporting) of genetic –> daughter cell
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Question 2

Each daughter cell contain one original(parental / template) , what we call this

Answer 2

Semiconservative

Question 3

In semiconservative
Template double helix is separated into ___ strand

Answer 3

2

Question 4

Name Prepriming complex and its function

Answer 4

1/ DNa A
-Main function is to recognize origin(بداية) of replication
- separate small region of parental double helix

2/helicase enzymes( لانه يفك الهلكس) (Dna B)
-separate double helix by cleavage of Hydrogen bond
3/ SSB
-prevent rejoining
- protect from nuclease
- prevent intra-chain H bond formation

Question 5

What is the another name of intra-chain H bond formation ?

Answer 5

Hairpin

Question 6

A child suffering from bloom disease, what is the issue?

Answer 6

Genetic defect in DNA helicase or unwinding protein

Question 7

What are the characteristics of bloom syndrome?

Answer 7

Short stature
Predisposition(قابلية) to cancer

Question 8

خذها القاعدة
When DNA is a single strand in the cell , it will be attacked by nuclease enzymes because it will think it is a antigen microbe DNA, what is the prepriming complex that protects our parental strand ?

Answer 8

Single strand binding

Question 9

Solve supercoils that is formed during unwinding , who is it

Answer 9

Topoisomerases

Question 10

Topoisomerases has both ____ that ____cutting & and ____ ligase ____ that _____ cutting

Answer 10

Nucleoase
Strand
Ligase
Reseal