DNA Replication

Question 1

DNA polymerase

Answer 1

An enzyme that catalyses formation of DNA from activated deoxyribose nucleotides, using single stranded DNA as a template

Question 2

Helicase

Answer 2

Enzyme that catalyses the breaking of hydrogen bonds between the nitrogenous pairs of bases in a DNA molecule

Question 3

Semi conservative replication

Answer 3

Two new molecules formed , each containing one old and one new strand. One old strand is conserved in each new molecule

Question 4

What enzyme unwinds the DNA?

Answer 4

The gyrase enzyme

Question 5

What enzyme unzip the DNA

Answer 5

Helicase

Question 6

What does DNA polymerase do?

Answer 6

Catalyses the addition of the new nucleotide bases in the 5’ to 3’ direction

Question 7

What dies the ligase enzyme do?

Answer 7

Joins fragmented lagging strand together

Question 8

What scientists conducted an experiment showing replication is semiconervative

Answer 8

Meselson and stahl

Question 9

What was the experiment for semiconservative replication ?

Answer 9

They grew e coli in 15N ( heavy)
Then left it in 14N untill it replicated
The DNA after one replication was hybrid DNA
They divided once more and one was hybrid one was light 15N