DNA libraries Flashcards

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1
Q

DNA libraries

A

Set of cloned DNA fragments from a single source or organism

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2
Q

Types of DNA libraries

A
  • Whole genome (genomic library)
  • Single chromosome (chromosome-specific library)
  • Gene/set of genes that are expressed in a single cell type or at a certain time (cDNA library – transcriptome
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3
Q

To make a genomic DNA library

A
  1. chromosomal DNA is extracted.
  2. It is digested with restriction enzymes, and the resulting fragments are inserted into vectors
  3. Vector selection: the library ideally contains the whole genome in the smallest number of clones.
  4. BACs and YACs used to accommodate the large sizes of DNA necessary to span the approximately 3 billion bp of human DNA (as in the Human Genome Project).
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4
Q

Slide 15-19 part 1 and slide 4-9 of RecDNA- Es2

A
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