DNA Lecture 1 Flashcards

1
Q

type of RNAs that are a component of

ribosomes

A

rRNAs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

type of RNAs that are intermediaries,
transmitting genetic information from the site of
DNA in a cell (primarily the nucleus) to the site of
protein synthesis in the cell (ribosome).

A

mRNAs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Type of RNAs that are adapter molecules,
translating the information in mRNA into a
specific amino acid sequence

A

tRNAs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

The repositories and
transmitters of genetic information for every
cell, tissue and organism

A

nucleic acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

nucleic acids are polymer chains with
similar monomers (called nucleotides) connected
by

A

covalent bonds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What are the three characteristic components of nucleotides

A

1) A phosphate group
2) A five-carbon sugar
3) A heterocyclic nitrogenous base

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What are the five carbon sugars in RNA and DNA?

A

beta-D-ribose in RNA

beta-2’-deoxy-D-ribose in DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

The molecule in RNA/DNA without the phosphate group is called the

A

nucleoside

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

The numbered carbon atoms of the ribose component in the nucleotides are given a prime (‘) designation to distinguish them from the numbered atoms of the

A

Nitrogenous base

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

In DNA nucleotides, the C-2 hydroxyl group of ribose in replaced by…(called 2-deoxy)

A

A hydrogen atom

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

refers to the stereochemical configuration of C-1 (anomeric carbon) in the cyclic form of 2-deoxy-D-ribose. Note that the OH on the C-1 is on the same side of the ring as C-5

A

β

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Refers to the stereochemical configuration of the C-4 (penultimate carbon). It has the same configuration as D-glyceraldehyde

A

D

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Ribose is an _____ because it has an aldehyde group

A

Aldose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

In _______, the straight chain (aldehyde) and ring (β-furanose) forms of free ribose are in equilibrium

A

Solution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

In _______, both types of pentoses are in their β-furanose form (closed-five membered ring)

A

Nucleotides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

n solution, all monosaccharides with five or more carbon atomsin the backbone occur predominantly as cyclic (ring) structures in which the carbonyl group forms a ____ with the oxygen of the hydroxyl group along the chain

A

covalent bond

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

The cyclic hemiacetal form that results from intramolecular reaction of the the C-4 OH group with the C-1 aldehyde group

A

D-ribose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

The anomeric carbon (C-1) is the

The chiral carbon that is most distant from the carbonyl carbon is the

A

Hemiacetal carbon

Penultimate carbon

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Is the pentose ring planar?

A

No, it has puckered conformations

20
Q

The bases in nucleotides can be characterized as ___ and _____

A

purines and pyrimidines

21
Q

Bases in nucleotides that are monocyclic nitrogen heterocycles

A

Pyrimidines

22
Q

Bases in nucleotides that are bicyclic nitrogen heterocycles

23
Q

Both DNA and RNA contain two major ____ bases:

A

Purine, Adenine (A) and Guanine (G)

24
Q

DNA and RNA also contain two major _____ bases

For DNA, they are

A

pyrimidine

cytosine and thymine

cytosine and uracil

25
Q

Why are purines and pyrimidines referred to as bases?

A

The NH2 and or ring nitrogen atoms are weakly basic, the lone pair of electrons on the nitrogen atom can accept a proton in an acid-base equilibrium

26
Q

Another characteristic of nucleotide bases is that they can exist in other ______ forms

There reaction equilibria favors the ____ and _____ forms (they are the most stable)

A

tautomeric

amino and keto

27
Q

Tautomeric forms of nucleotide bases have different ______ characteristics

28
Q

N-1 of adenine is an H-bond _____ in the amino form, but an H-bond ____ in the imino form

A

acceptor

donor

29
Q

The keto oxygen on C-4 of thymine can as as an H-bond ______ while the nol form can act as an H bond ______

A

acceptor

donor

30
Q

Why is hydrogen bonding so important in nucleotide bases?

A

H-bonding of amino and keto forms of the bases allows a complimentary association of two strands of DNA

31
Q

What are the complimentary base pairs in DNA and RNA?

A

DNA: A-T , C-G

RNA A-U, C-G

32
Q

Since the imino form of A can’t form H-bonds with the keto form of T in the same way, spontaeneous ___ can result from the formation of abnormal base pairs

33
Q

The purines and pyrimidines are what kind of molecules?

34
Q

Electron delocatlization among ring atoms or purines and pyrimidines results in most of the bonds having partial…

A

double bound character

35
Q

Pyrimidines are nearly planar/planar

purines are nearly planar/planar

A

planar

nearly planar

36
Q

Are purines and pyrdimidines hydrophilic or hydrophobic?

Are they soluble or insoluble in water at the cell pH (near neutral)?

A

Hydrophobic

insolube

37
Q

Bases are covalently bonded to ribose or deoxyribose in an N-β - glycosyl bond to the ___ carbon of the pentose ring

38
Q

The bonding of bases to ribose or deoxyribose occurs where on pyrimidines?

Where on purines?

A

N-1 of pyrdimidines

N-9 of purines

39
Q

The N-β-glycosyl bond is formed by removal of the elements of..

The resulting structure (ribose or deoxyribose with base attached) is called a

A

Water (OH from pentose and H from heterocyclic base)

Nucleoside

40
Q

When a phosphate is _______ to the 5’ carbon of the pentose ring with base attached, the resulting structure is a

A

esterified, nucleotide

41
Q

The nucleotides are linked together by ____ t0 _____ phosphodiester bonds to form a linear polmer

A

5’ to 3’ phosphodiester bonds

42
Q

The covalent backbones of nucleic acids consist of alternating phosphate and pentose residues

The nitrogenous bases may be thought of as side groups joined to the backbone at regular intervals

43
Q

The polymer chain at the terminal nucleotide at the 5’ end has a free ________ group

The terminal group at the 3’ end has a free

A

5’ phosphate group

3’ OH group

44
Q

When the phosphate group forms a diester linkage, there’s on ionizible oxygen shown in the ionized form. Why is phosphodiester in the ionized form?

A

Phosphoric acid is a tribasic acid that has 3 ionization equilibria…

The ionization of the phosphodiester group found in a nucleic acid corresponds to a pKa ~ 1.4

Based on this pKa, the unionized form of a polymer of nucleotides would be a strong acid

45
Q

The ionized form of a polymer of nucleotides with the negative charge on the phosphate oxygen will exist almost exclusively at pH =