dna extraction Flashcards
1
Q
describe the method of DNA extraction
A
place a piece of fruit in a beaker and crush it
add detergent and salt, mix
filter the mixture and collect the liquid in a test tube
pour chilled ethanol into the test tube
DNA precipitates forming a fibrous white solid
use a glass rod to collect the DNA sample
2
Q
why is salt added to the crushed fruit?
A
salt encourages the precipitation of DNA
3
Q
glass rod
A
collect/remove the DNA
4
Q
crushing the fruit
A
helps access cell content
5
Q
water bath
A
denatures the enzyme
6
Q
salt water
A
clumps DNA together
7
Q
washing up liquid
A
breaks down the cell membranes
