DNA and the genome Flashcards
DNA (Deoxyribonucleic Acid)
The molecule that contains the genetic instructions for life. It is composed of nucleotides and forms a double helix structure.
base pairing
The hydrogen bonding between specific nitrogenous bases in DNA. Adenine pairs with thymine (A-T), and guanine pairs with cytosine (G-C).
nucleotide
The building block of DNA, consisting of a deoxyribose sugar, a phosphate group, and a nitrogenous base (adenine, thymine, guanine, cytosine).
sugar-Phosphate Backbone
The structural framework of DNA, formed by linked sugar and phosphate groups of nucleotides, running along the DNA strand.
Double-Stranded Antiparallel Structure
The orientation of the two DNA strands running in opposite directions (5’ to 3’ and 3’ to 5’).
double helix
The twisted ladder-like shape formed by two strands of DNA.
prokaryote
A unicellular organism that lacks a nucleus. Its DNA is organized in a single circular chromosome and sometimes plasmids.
Eukaryote
An organism whose cells have a nucleus enclosed within membranes, with linear chromosomes in the nucleus and circular chromosomes in mitochondria and chloroplasts.
plasmid
A small, circular DNA molecule found in prokaryotes and some eukaryotes, like yeast, which can replicate independently.
linear chromosome
The structure found in eukaryotic cells that contains tightly coiled DNA packaged with proteins.
circular chromosome
DNA that is circular in shape, found in prokaryotes, mitochondria, chloroplasts, and some eukaryotes like yeast.
DNA Replication
The process by which a DNA molecule is copied to produce two identical DNA molecules.
DNA Polymerase
An enzyme that adds nucleotides to the growing DNA strand during DNA replication, using complementary base pairing.
primer
A short strand of RNA or DNA that serves as a starting point for DNA synthesis.
DNA Ligase
An enzyme that joins DNA fragments together by forming phosphodiester bonds.
Polymerase Chain Reaction (PCR)
A technique used to amplify specific DNA sequences through repeated cycles of heating and cooling.
Primer in PCR
Short DNA sequences that initiate DNA synthesis at specific target regions during PCR.
Gene Expression
The process by which information from a gene is used to synthesize proteins, involving transcription and translation.
Transcription
The process of copying a DNA sequence into messenger RNA (mRNA).
Translation
The process where mRNA is decoded by ribosomes to produce a specific protein.
Messenger RNA (mRNA)
RNA that carries genetic information from DNA in the nucleus to the ribosome, where it is translated into a protein.
Transfer RNA (tRNA)
RNA that carries specific amino acids to the ribosome during protein synthesis, matching its anticodon with the mRNA codon.
Ribosomal RNA (rRNA)
RNA that, together with proteins, makes up the ribosome and facilitates protein synthesis.
RNA Polymerase
An enzyme that synthesizes RNA from a DNA template during transcription.
Primary mRNA Transcript
The initial RNA transcript produced by transcription, containing both exons and introns.
RNA Splicing
The process by which introns are removed from the primary mRNA transcript, and exons are joined together to form mature mRNA.
Intron
A non-coding region of an RNA transcript or the DNA encoding it, removed during RNA splicing.
Exon
A coding region of DNA or RNA that is retained after RNA splicing and is translated into protein.
Mature mRNA Transcript
The processed RNA molecule, after splicing, that contains only exons and is ready for translation.
Anticodon
A sequence of three nucleotides in tRNA that is complementary to a codon in mRNA, enabling the tRNA to deliver the correct amino acid during translation.
Codon
A sequence of three nucleotides in mRNA that specifies a particular amino acid or a start/stop signal during translation.
Peptide Bond
The chemical bond that links amino acids together in a protein.