DNA and Biotech

Question 1

What structural feature contributes the high energy feature of Adenosine Triphosphate

Answer 1

The negatively charged phosphate that repel each other.

Question 2

After hybridizing 2 different stands of DNA, how could you verify the strands have annealed

Answer 2

Measure UV light absorption difference between single strand and the newly formed double strand.

Question 3

what is allolactose

Answer 3

a disaccharide that engenders a conformational change in the repressor protein, which causes this protein to disassociate from the lac operon, allowing for transcription.

Question 4

what is a role of an activator

Answer 4

to enhance the interaction between the promoter region and RNA polymerase, usually by binding a promoter-proximal element.

Question 5

Function of the 5’ cap of mRNA

Answer 5

Regulate Nuclear Export
Protect RNA from degradation
Promote ribosome binding.

Question 6

Purpose of microRNA

Answer 6

to cause repression or degradation of the target through mRNA binding.