DNA Flashcards

1
Q

microbiology is:

A

the study of the interactions and regulation of various systems of a cell, including interactions bw DNA, RNA, and proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

6 basics of life:

A

metabolism, growth, reproduction, genetic variation/evolution, response/adaptation to exterior environment, homeostasis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

4 essential macromolecules for life

A

polypeptides (proteins when big enough), nucleic acids, lipids, polysaccharides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

ribozymes

A

polypeptides as enzymes. they are important for metabolism, replication, etc. they act as structural proteins for cells.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

central dogma of biology

A

the coded genetic information hard-wired into DNA is transcribed into individual transportable cassettes, composed of mRNA. each mRNA cassette contains the program for synthesis of a particular protein

DNA replicated into two copies. DNA transcribed into mRNA. RNA translated into proteins.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

microbiologist’s tools

A

techniques used to measure and evaluate DNA, RNA, and proteins. microbiologist weigh and measure and compare the differences

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

DNA vs. Genes

A

we all have the same set f genes, but different DNA within the gene.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

gene definition:

A

a locatable region of genomic sequence, corresponding to a unit of inheritance, which is associated with regulatory regions, transcribed regions, and/or other functional sequence regions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Is science fact?

A

no, it is hypothesis based

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

all cells in the body have the same DNA except…

A

red blood cells, gametes, cancer cells, T & B cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

how much DNA do humans share with one another?

A

~99%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

DNA take homes:

A

small changes make a big difference/ most molecular materials are highly conserved

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

mutation cause:

A

changes in protein levels or function

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

allele:

A

“different flavors of genes”

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

why does elongation occur in the 5’-3’ directions?

A

elongation is a Sn2 attack, and the action can only occur down the bonds in the 5’-3’ direction.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

H-bonding in nucleotides

A

A-T=2 bonds

G-C=3 bonds

17
Q

How to replicate DNA in a test tube

A
  1. Heat/denature the DNA
  2. Taq polymerase (PCR)
  3. use of synthesized/designed primers
18
Q

PCR

A

polymerase chain reaction
reaction is run 35x to make about 34 billion copies
**base pairing runs in the 5’-3’ direction and goes from the 5’ primer to the end of the 3’ primer